Grape ASR Regulates Glucose Transport, Metabolism and Signaling
MEDICI, Anna
Écologie et biologie des interactions [EBI [Poitiers]]
Institut des Sciences des Plantes de Montpellier [IPSIM]
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Écologie et biologie des interactions [EBI [Poitiers]]
Institut des Sciences des Plantes de Montpellier [IPSIM]
MEDICI, Anna
Écologie et biologie des interactions [EBI [Poitiers]]
Institut des Sciences des Plantes de Montpellier [IPSIM]
Écologie et biologie des interactions [EBI [Poitiers]]
Institut des Sciences des Plantes de Montpellier [IPSIM]
VERBEKE, Jérémy
Écologie et biologie des interactions [EBI [Poitiers]]
Génétique, Reproduction et Développement [GReD]
< Réduire
Écologie et biologie des interactions [EBI [Poitiers]]
Génétique, Reproduction et Développement [GReD]
Langue
en
Article de revue
Ce document a été publié dans
International Journal of Molecular Sciences. 2022, vol. 23, n° 11, p. 6194
MDPI
Résumé en anglais
To decipher the mediator role of the grape Abscisic acid, Stress, Ripening (ASR) protein, VvMSA, in the pathways of glucose signaling through the regulation of its target, the promoter of hexose transporter VvHT1, we ...Lire la suite >
To decipher the mediator role of the grape Abscisic acid, Stress, Ripening (ASR) protein, VvMSA, in the pathways of glucose signaling through the regulation of its target, the promoter of hexose transporter VvHT1, we overexpressed and repressed VvMSA in embryogenic and non-embryogenic grapevine cells. The embryogenic cells with organized cell proliferation were chosen as an appropriate model for high sensitivity to the glucose signal, due to their very low intracellular glucose content and low glycolysis flux. In contrast, the non-embryogenic cells displaying anarchic cell proliferation, supported by high glycolysis flux and a partial switch to fermentation, appeared particularly sensitive to inhibitors of glucose metabolism. By using different glucose analogs to discriminate between distinct pathways of glucose signal transduction, we revealed VvMSA positioning as a transcriptional regulator of the glucose transporter gene VvHT1 in glycolysis-dependent glucose signaling. The effects of both the overexpression and repression of VvMSA on glucose transport and metabolism via glycolysis were analyzed, and the results demonstrated its role as a mediator in the interplay of glucose metabolism, transport and signaling. The overexpression of VvMSA in the Arabidopsis mutant abi8 provided evidence for its partial functional complementation by improving glucose absorption activity.< Réduire
Mots clés
Fruit
Raisin
Métabolisme
Mots clés en anglais
ASR
VvMSA overexpression/repression
VvHT1
grape embryogenic and non-embryogenic cells
glucose signaling
glucose absorption
sugar transporters
central metabolism
Arabidopsis abi8 mutant
ABA/glucose sensitivity
germination
Project ANR
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
Origine
Importé de halUnités de recherche