Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description
LEBAS, Benedicte
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
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Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
LEBAS, Benedicte
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
BLOUIN, Arnaud
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
CHANDELIER, Anne
Centre wallon de Recherches Agronomiques [Belgique] = Walloon Agricultural Research Centre [Belgium] [CRA-W]
Centre wallon de Recherches Agronomiques [Belgique] = Walloon Agricultural Research Centre [Belgium] [CRA-W]
HURTADO-GONZALES, Oscar
Animal Plant Health Inspection Service, APHIS-USDA, Humble, Texas, 77338, USA.
Animal Plant Health Inspection Service, APHIS-USDA, Humble, Texas, 77338, USA.
MACLOT, François
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
MINAFRA, Angelantonio
CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP]
CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP]
PETTER, Françoise
European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes [EPPO]
European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes [EPPO]
PONCHART, Julien
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
ROLLIN, Johan
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
SALDARELLI, Pasquale
CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP]
CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP]
TAMISIER, Lucie
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
TRONTIN, Charlotte
European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes [EPPO]
European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes [EPPO]
VICENTE, Claudia
Instituto Nacional de Investigação Agrária e Veterinária = National Institute for Agrarian and Veterinary Research [Oeiras, Portugal] [INIAV]
Instituto Nacional de Investigação Agrária e Veterinária = National Institute for Agrarian and Veterinary Research [Oeiras, Portugal] [INIAV]
MASSART, Sebastien
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
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Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Langue
en
Article de revue
Ce document a été publié dans
EPPO Bulletin. 2022, vol. 52, n° 2, p. 394-418
Wiley
Résumé en anglais
High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for ...Lire la suite >
High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories for performing HTS testing. This paper describes general and technical recommendations to guide laboratories through the complex process of preparing a laboratory for HTS tests within existing quality assurance systems. From nucleic acid extractions to data analysis and interpretation, all of the steps are covered to ensure reliable and reproducible results. These guidelines are relevant for the detection and identification of any plant pest (e.g. arthropods, bacteria, fungi, nematodes, invasive plants or weeds, protozoa, viroids, viruses), and from any type of matrix (e.g. pure microbial culture, plant tissue, soil, water), regardless of the HTS technology (e.g. amplicon sequencing, shotgun sequencing) and of the application (e.g. surveillance programme, phytosanitary certification, quarantine, import control). These guidelines are written in general terms to facilitate the adoption of HTS technologies in plant pest routine diagnostics and enable broader application in all plant health fields, including research. A glossary of relevant terms is provided among the Supplementary Material.< Réduire
Mots clés en anglais
high-throughput sequencing
plant pest diagnostic test
Projet Européen
Validation of diagnostic tests to support plant health
Origine
Importé de halUnités de recherche