BOURAFAI-AZIEZ, Asma; JACOB, Daniel; CHARPENTIER, Gwladys; CASSIN, Emmanuel; ROUSSELOT, Guillaume; MOING, Annick; DEBORDE, Catherine

doi:10.3390/molecules27175614

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BOURAFAI-AZIEZ, Asma
Evear Extraction

JACOB, Daniel
Biologie du fruit et pathologie [BFP]

CHARPENTIER, Gwladys
Evear Extraction

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Article de revue

Ce document a été publié dans

Molecules. 2022, vol. 27, n° 17, p. 5614

MDPI

Résumé en anglais

Acerola (Malpighia emarginata D.C.) is an exotic fruit with high agro-industrial potential due to its high content of ascorbic acid (AA), phenolic compounds, and carotenoid pigments. Acerola fruit is processed into concentrated juice or powder to be incorporated into food supplements. The ascorbic acid content of concentrated juice or powders must be controlled and well assessed. Therefore, the development of optimal methods and procedures for the rapid and accurate determination of the ascorbic acid content in juice concentrate and juice powder remains of considerable commercial interest. NMR spectroscopy is currently a powerful spectroscopic tool for the qualitative and quantitative analysis of molecules of all types and sizes. Firstly, this article presents the NMR-based metabolomic profiling of acerola juice and concentrate powder to describe and compare their composition. Thirty-six metabolites were identified. The AA over choline ratio and the NMR metabolomic profiles could be used for authentication in the future. Secondly, a rapid (8 min), reliable, and non-destructive method for the quantification of ascorbic acid by 1D 1H-NMR spectroscopy was developed and validated. The LOD and LOQ were 0.05 and 0.15 mg/mL, respectively. These two approaches could be combined to better characterize ingredients derived from acerola and incorporated into food supplements.< Réduire

Mots clés en anglais

acerola

metabolomic profiling

ascorbic acid

vitamin C

qNMR

food supplement

Malpighia emarginata D.C

DOI

http://dx.doi.org/10.3390/molecules27175614

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

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Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332