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hal.structure.identifierLaboratoire Joliot Curie
dc.contributor.authorBERGUIGA, Lotfi
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorSTREPPA, Laura
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorBOYER-PROVERA, Elise
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorMARTINEZ-TORRES, Cristina
hal.structure.identifierLaboratoire de Biologie Moléculaire de la Cellule [LBMC]
dc.contributor.authorSCHAEFFER, Laurent
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorELEZGARAY, Juan
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARNEODO, Alain
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorARGOUL, Françoise
dc.date.created2015-11-13
dc.date.issued2016-02-20
dc.identifier.issn1559-128X
dc.description.abstractEnWe report on a fibered high-resolution scanning surface plasmon microscope for long term imaging of living adherent cells. The coupling of a high numerical aperture objective lens and a fibered heterodyne interferometer enhances both the sensitivity and the long term stability of this microscope, allowing for time-lapse recording over several days. The diffraction limit is reached with a radially polarized illumination beam. Adherence and motility of living C2C12 myoblast cells are followed for 50 h, revealing that the dynamics of these cells change after 10 h. This plasmon enhanced evanescent wave microscopy is particularly suited for investigating cell adhesion, since it can not only be performed without staining of the sample but it can also capture in real time the exchange of extracellular matrix elements between the substrate and the cells.
dc.language.isoen
dc.publisherOptical Society of America
dc.rights.urihttp://creativecommons.org/licenses/by-sa/
dc.subject.enSurface plasmons
dc.subject.enScanning microscopy
dc.subject.enMedical and biological imaging
dc.subject.enInterferometry
dc.subject.enFiber optics
dc.subject.enNoise in imaging systems
dc.title.enTime-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam
dc.typeArticle de revue
dc.identifier.doi10.1364/AO.55.001216.v001
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
bordeaux.journalApplied optics
bordeaux.page1216-1227
bordeaux.volume55
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-01279128
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01279128v1
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