Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam
ARNEODO, Alain
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
ARGOUL, Françoise
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
< Réduire
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Langue
en
Article de revue
Ce document a été publié dans
Applied optics. 2016-02-20, vol. 55, n° 6, p. 1216-1227
Optical Society of America
Résumé en anglais
We report on a fibered high-resolution scanning surface plasmon microscope for long term imaging of living adherent cells. The coupling of a high numerical aperture objective lens and a fibered heterodyne interferometer ...Lire la suite >
We report on a fibered high-resolution scanning surface plasmon microscope for long term imaging of living adherent cells. The coupling of a high numerical aperture objective lens and a fibered heterodyne interferometer enhances both the sensitivity and the long term stability of this microscope, allowing for time-lapse recording over several days. The diffraction limit is reached with a radially polarized illumination beam. Adherence and motility of living C2C12 myoblast cells are followed for 50 h, revealing that the dynamics of these cells change after 10 h. This plasmon enhanced evanescent wave microscopy is particularly suited for investigating cell adhesion, since it can not only be performed without staining of the sample but it can also capture in real time the exchange of extracellular matrix elements between the substrate and the cells.< Réduire
Mots clés en anglais
Surface plasmons
Scanning microscopy
Medical and biological imaging
Interferometry
Fiber optics
Noise in imaging systems
Origine
Importé de halUnités de recherche