MARIETTE, Stéphanie; WONG JUN TAI, Fabienne; ROCH, Guillaume; BARRÉ, Aurélien; CHAGUE, Aurélie; DECROOCQ, Stéphane; GROPPI, Alexis; LAIZET, Yec’han; LAMBERT, Patrick; TRICON, David; NIKOLSKI, Macha; AUDERGON, Jean-Marc; ABBOTT, Albert; DECROOCQ, Véronique

doi:10.1111/nph.13627

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MARIETTE, Stéphanie
from patterns to models in computational biodiversity and biotechnology [PLEIADE]
Biologie du fruit et pathologie [BFP]

WONG JUN TAI, Fabienne
Centre de Bioinformatique de Bordeaux [CBIB]

ROCH, Guillaume
CEP Innovation

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Ce document a été publié dans

New Phytologist. 2016, vol. 209, n° 2, p. DOI: 10.1111/nph.13627

Wiley

Résumé en anglais

SummaryIn fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. However, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. To address this problem for Plum Pox Virus (PPV) candidate resistance gene identification in Prunus species, we implemented a genome-wide association (GWA) approach in apricot.This study exploited the broad genetic diversity of the apricot (Prunus armeniaca) germplasm containing resistance to PPV, next-generation sequence-based genotyping, and the high-quality peach (Prunus persica) genome reference sequence for single nucleotide polymorphism (SNP) identification.The results of this GWA study validated previously reported PPV resistance quantitative trait loci (QTL) intervals, highlighted other potential resistance loci, and resolved each to a limited set of candidate genes for further study.This work substantiates the association genetics approach for resolution of QTL to candidate genes in apricot and suggests that this approach could simplify identification of other candidate genes for other marked trait intervals in this germplasm.< Réduire

Mots clés

prunus species

Mots clés en anglais

candidate genes

genome-wide association study (GWAS)

genome-wide resequencing

sharka

single nucleotide polymorphism (SNP)

plum pox virus (PPV) resistance

apricot