Phytotechnologies are a set of sustainable, ecological options notably for alleviating pollutant linkages inducedby contaminated soils. However, concerns exist regarding the processing of trace elements (TE)-enrichedbiomass and the fate of TEs in the end-products. The fractionation of phytoremediation TE-enriched non-woodylignocellulosic (tobacco) and woody (birch, willow) phytoremediation-borne biomasses was studied usingethanol organosolv, soda and dilute acid pretreatments. TE distribution in the process fractions (pulp, liquideffluents and lignin) was further examined. In dilute acid conditions, a wood pretreatment performed at 170 °Cin the presence of 2% w/w of sulfuric acid allowed an efficient extraction of the metals in the water effluent(≈90% for Mn and Zn) producing a clean pulp. A soda pretreatment resulted in a low metal extractability,especially at high temperature. At 170 °C or above with 15% w/w of NaOH, metals were mostly recovered in thecellulosic pulp (70%- 98%). Using organosolv pulping, the TE contents were in the decreasing order:pulp ≫ water stream > lignin. The TE organosolv extraction in the liquid phase varied depending on the TEmetal and on the species but was generally low. Metal extractability increased with the water content in thesolvent of the organosolv pulping but decreased with temperature. Enzymatic hydrolyses of TE-enriched andmetal-free pulps were investigated and it was concluded that the TE concentrations had little or no effect onpolysaccharide enzymatic hydrolysability by cellulases. The final destination of the various fractions forvalorization in a biorefinery setting is discussed, regarding their TE content.< Réduire