BOUTEILLER, Xavier P.; PORTE, Annabel J.; MARIETTE, Stéphanie; MONTY, Arnaud

doi:10.1017/S0960258517000150

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http://creativecommons.org/licenses/by-sa/

BOUTEILLER, Xavier P.
Biodiversité, Gènes & Communautés [BioGeCo]

PORTE, Annabel J.
Biodiversité, Gènes & Communautés [BioGeCo]

MARIETTE, Stéphanie
Biodiversité, Gènes & Communautés [BioGeCo]

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Article de revue

Ce document a été publié dans

Seed Science Research. 2017, vol. online first, p. 1-8

Cambridge University Press (CUP)

Résumé en anglais

Physical dormancy of Robinia pseudoacacia seeds makes it a challenge for scientists and forest managers to obtain a homogeneous germination for larger seed samples. Water imbibition of the seeds can be achieved through manual piercing of the seed coat, but this method remains time consuming and heterogeneous. We tested several ecologically friendly methods to break seed dormancy, including manual pin puncture, water soaking, oven dry-heating (two temperatures) and sanding. Sanding was performed using an automatic grinder to control shaking duration (three durations) and get a homogeneous scraping of the coat. All methods, except dry-heating, resulted in successful dormancy breaking; water soaking was the least efficient method, attaining 57% germination. Sanding proved to be as efficient as puncturing (97%) but long duration sanding (10 or 15 min) could damage cotyledons, which would impede further development of the plant. Short-time sanding (5 min) proved to be the best method to reach high total germination and healthy (undamaged cotyledon) seedlings, and was successfully applied to 500 seeds. The reference puncture method and the automatic sanding were also tested on seeds of nine Fabaceae species and proved to be efficient for some species. Automated sanding can thus be used as a standard to break physical dormancy of black locust or other Fabaceae seeds to allow further comparative studies of plant populations or genotypes.< Réduire

Mots clés

fabaceae

scarification

robinia pseudoacacia

dormance

Mots clés en anglais

automated grinder

dormancy breaking

germination

sanding

standard protocol

locust trees

dormancy

DOI

http://dx.doi.org/10.1017/S0960258517000150

Project ANR

COntinental To coastal Ecosystems: evolution, adaptability and governance - ANR-10-LABX-0045

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202