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hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorJORGE, Véronique
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorDUPLESSIS, Sébastien
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorBRESSON, Alois
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorDOWKIW, Arnaud
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorFAIVRE-RAMPANT, Patricia
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorFREY, Pascal
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorGUÉRIN, Vanina
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorKOHLER, Annegret
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorMARTIN, Francis
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorPAOLUCCI, Isabella
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorRINALDI, Cécile
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorBASTIEN, Catherine
dc.date.issued2009
dc.date.conference2009-07-05
dc.description.abstractEnFoliar rust is the most devastating disease affecting European poplar plantations. One major QTL (Rus), identified in a Populus deltoides x P. trichocorpa pedigree, is on the process of positional cloning. To precise the molecular basis of Rus, joint proteomic and transcriptomic approaches were used to characterize differential expression 2 and 4 days post inoculation (dpi) in two bulks (Rus vs rus) of 10 progenies. At 2 dpi, few transcripts showed an altered expression in the Rus bulk, while genes known to be targeted by resistance genes in plant pathosystems, were found up-regulated 4 dpi. The rus bulk did not present such gene up-regulation while several transcripts, including defense-related genes, were down-regulated as early as 2 dpi. Changes in the proteome were studied by 2-D PAGE analysis and MS/MS characterization of a selected subset of 50 protein spots at 2 and 4 dpi. Interestingly, the proteins overexpressed in the Rus genotypes correlated quite well with the transcriptomic data. All the results, including data coming from positional cloning, suggested that Rus activates the same genes than those involved in qualitative resistance, but this activation is delayed in the quantitative response.
dc.language.isoen
dc.subjectQUANTITATIVE RESISTANCE
dc.subjectTRANSCRIPTOMICS
dc.subjectPROTEOMICS
dc.subject.enFOLIAR RUST
dc.subject.enPOPLAR RUST
dc.title.enJoint transcriptomic and proteomic approaches to precise the molecular basis of a major QTL (RUS) controlling quantitative rust resistance in poplar
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
bordeaux.conference.titlePlant genomics and beyond
bordeaux.countryFR
bordeaux.conference.cityEvry
bordeaux.peerReviewednon
hal.identifierhal-02756173
hal.version1
hal.invitedoui
hal.conference.end2009-07-08
hal.popularnon
hal.audienceNationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02756173v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2009&rft.au=JORGE,%20V%C3%A9ronique&DUPLESSIS,%20S%C3%A9bastien&LALANNE,%20C%C3%A9line&BRESSON,%20Alois&DOWKIW,%20Arnaud&rft.genre=unknown


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