MOUSSEAU, Fanny; OIKONOMOU, Evdokia; VACHER, Annie; AIRIAU, Marc; MORNET, Stéphane; BERRET, Jean-François

doi:10.1039/C9NA00779B

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MOUSSEAU, Fanny
Matière et Systèmes Complexes [MSC]

OIKONOMOU, Evdokia
Matière et Systèmes Complexes [MSC]

VACHER, Annie

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Article de revue

Ce document a été publié dans

Nanoscale Advances. 2020-02-18, vol. 2, n° 2, p. 642-647

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Résumé en anglais

When inhaled, nanoparticles (NPs) deposit in alveoli and transit through the pulmonary surfactant (PS), a biofluid made of proteins and phospholipid vesicles. They form a corona reflecting the PS–nanomaterial interaction. Since the corona determines directly the NPs' biological fate, the question of its nature and structure is central. Here, we report on the corona architecture formed after incubation of positive or negative silica particles with Curosurf®, a biomimetic pulmonary surfactant of porcine origin. Using optical, electron and cryo-electron microscopy (cryo-TEM), we determine the pulmonary surfactant corona structure at different scales of observation. Contrary to common belief, the PS corona is not only constituted by phospholipid bilayers surrounding NPs but also by multiple hybrid structures derived from NP–vesicle interaction. Statistical analysis of cryo-TEM images provides interesting highlights about the nature of the corona depending on the particle charge. The influence of Curosurf® pre- or post-treatment is also investigated and demonstrates the need for protocol standardization.< Réduire

Mots clés en anglais

Cite this: Nanoscale Adv

2020

642

DOI

http://dx.doi.org/10.1039/C9NA00779B

Project ANR

Interactions des nanoparticules avec des surfaces mimétiques des poumons et du liquide pulmonaire - ANR-12-CHEX-0011

Origine

Importé de hal

Unités de recherche

Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) - UMR 5026