JACOBIN-VALAT, Marie-Josée; LAROCHE-TRAINEAU, Jeanny; LARIVIÈRE, Mélusine; MORNET, Stéphane; SANCHEZ, Stephane; BIRAN, Marc; LEBARON, Caroline; BOUDON, Julien; LACOMME, Sabrina; CÉRUTTI, Martine; CLOFENT-SANCHEZ, Gisèle

doi:10.1016/j.nano.2014.12.006

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JACOBIN-VALAT, Marie-Josée
Centre de résonance magnétique des systèmes biologiques [CRMSB]
Plateforme Technologique et d'Innovation Biomédicale

LAROCHE-TRAINEAU, Jeanny
Centre de résonance magnétique des systèmes biologiques [CRMSB]
Plateforme Technologique et d'Innovation Biomédicale

LARIVIÈRE, Mélusine
Centre de résonance magnétique des systèmes biologiques [CRMSB]
Plateforme Technologique et d'Innovation Biomédicale

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Article de revue

Ce document a été publié dans

Nanomedicine: Nanotechnology, Biology and Medicine. 2015, vol. 11, n° 4, p. 927-937

Elsevier

Résumé en anglais

Atherosclerosis is an inflammatory disease associated with the formation of atheroma plaques likely to rupture in which platelets are involved both in atherogenesis and atherothrombosis. The rupture is linked to the molecular composition of vulnerable plaques, causing acute cardiovascular events. In this study we propose an original targeted contrast agent for molecular imaging of atherosclerosis. Versatile USPIO (VUSPIO) nanoparticles, enhancing contrast in MR imaging, were functionalised with a recombinant human IgG4 antibody, rIgG4 TEG4, targeting human activated platelets. The maintenance of immunoreactivity of the targeted VUSPIO against platelets was confirmed in vitro by flow cytometry, transmission electronic and optical microscopy. In the atherosclerotic ApoE(-/-) mouse model, high-resolution ex vivo MRI demonstrated the selective binding of TEG4-VUSPIO on atheroma plaques. It is noteworthy that the rationale for targeting platelets within atherosclerotic lesions is highlighted by our targeted contrast agent using a human anti-αIIbβ3 antibody as a targeting moiety. Current clinical assessment of atherosclerotic plagues is suboptimal. The authors in the article designed functionalized superparamagnetic iron oxide nanoparticles with TEG4, a recombinant human antibody, to target activated platelets. By using MRI, these nanoparticles can be utilized to study the process of atheroma pathogenesis.< Réduire

Mots clés en anglais

Human antibody

Atherosclerosis

Platelets

Nanoparticles

MRI contrast agents

DOI

http://dx.doi.org/10.1016/j.nano.2014.12.006

Project ANR

Translational Research and Advanced Imaging Laboratory - ANR-10-LABX-0057

Origine

Importé de hal

Unités de recherche

Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) - UMR 5026