CARIO, Anaïs; LARZILLIÈRE, Marina; NGUYEN, Olivier; ALAIN, Karine; MARRE, Samuel

doi:10.3389/fmicb.2022.866681

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CARIO, Anaïs
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]

LARZILLIÈRE, Marina
Biologie et Ecologie des Ecosystèmes Marins Profonds [BEEP]

NGUYEN, Olivier
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]

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Frontiers in Microbiology. 2022, vol. 13, p. 866681 (15 p.)

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Here, we present a novel methodology based on high-pressure microfluidics to rapidly perform temperature-based phenotyping of microbial strains from deep-sea environments. The main advantage concerns the multiple on-chip temperature conditions that can be achieved in a single experiment at pressures representative of the deep-sea, overcoming the conventional limitations of large-scale batch metal reactors to conduct fast screening investigations. We monitored the growth of the model strain Thermococcus barophilus over 40 temperature and pressure conditions, without any decompression, in only 1 week, whereas it takes weeks or months with conventional approaches. The results are later compared with data from the literature. An additional example is also shown for a hydrogenotrophic methanogen strain ( Methanothermococcus thermolithotrophicus ), demonstrating the robustness of the methodology. These microfluidic tools can be used in laboratories to accelerate characterizations of new isolated species, changing the widely accepted paradigm that high-pressure microbiology experiments are time-consuming.< Réduire

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high-pressure microfluidics

deep-sea microorganisms

real time investigations

phenotyping

fast screening

high-pressure microfluidics

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High-Pressure Microfluidics for Ultra-Fast Microbial Phenotyping

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