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Primary cilium migration depends on G-protein signalling control of subapical cytoskeleton

dc.rights.licenseopenen_US
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorEZAN, Jerome
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorLASVAUX, Lea
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorGEZER, Aysegul
dc.contributor.authorNOVAKOVIC, Ana
dc.contributor.authorMAY-SIMERA, Helen
dc.contributor.authorBELOTTI, Edwige
dc.contributor.authorLHOUMEAU, Anne-Catherine
dc.contributor.authorBIRNBAUMER, Lutz
dc.contributor.authorBEER-HAMMER, Sandra
dc.contributor.authorBORG, Jean-Paul
dc.contributor.authorLE BIVIC, Andre
dc.contributor.authorNÜRNBERG, Bernd
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorSANS, Nathalie
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorMONTCOUQUIOL, Mireille
dc.date.accessioned2022-07-01T12:32:02Z
dc.date.available2022-07-01T12:32:02Z
dc.date.issued2013-09
dc.identifier.issn1465-7392en_US
dc.identifier.urihttps://hal.archives-ouvertes.fr/hal-00862161
dc.identifier.uriftunivtuebing:oai:publikationen.uni-tuebingen.de:10900/41812
dc.identifier.uriftunivaixmarseil:oai:HAL:hal-00862161v1
dc.identifier.urioai:researchgate.net:255735548
dc.identifier.urioai:crossref.org:10.1038/ncb2819
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/140358
dc.description.abstractEnIn ciliated mammalian cells, the precise migration of the primary cilium at the apical surface of the cells, also referred to as translational polarity, defines planar cell polarity (PCP) in very early stages. Recent research has revealed a co-dependence between planar polarization of some cell types and cilium positioning at the surface of cells. This important role of the primary cilium in mammalian cells is in contrast with its absence from Drosophila melanogaster PCP establishment. Here, we show that deletion of GTP-binding protein alpha-i subunit 3 (Gαi3) and mammalian Partner of inscuteable (mPins) disrupts the migration of the kinocilium at the surface of cochlear hair cells and affects hair bundle orientation and shape. Inhibition of G-protein function in vitro leads to kinocilium migration defects, PCP phenotype and abnormal hair bundle morphology. We show that Gαi3/mPins are expressed in an apical and distal asymmetrical domain, which is opposite and complementary to an aPKC/Par-3/Par-6b expression domain, and non-overlapping with the core PCP protein Vangl2. Thus G-protein-dependent signalling controls the migration of the cilium cell autonomously, whereas core PCP signalling controls long-range tissue PCP.
dc.description.sponsorshipCaractérisation moléculaire et fonctionnelle de vangl2 et de ses partenaires chez le mammifèreen_US
dc.language.isoENen_US
dc.sourcebase
dc.sourceresearchgate
dc.sourcecrossref
dc.title.enPrimary cilium migration depends on G-protein signalling control of subapical cytoskeleton
dc.title.alternativeNat Cell Biol.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/ncb2819en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed23934215en_US
bordeaux.journalNature Cell Biologyen_US
bordeaux.page1107-1115en_US
bordeaux.volume15en_US
bordeaux.hal.laboratoriesNeurocentre Magendie - UMR-S 1215en_US
bordeaux.issue9en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDConseil Régional Aquitaineen_US
bordeaux.identifier.funderIDFondation pour la Recherche Médicaleen_US
bordeaux.identifier.funderIDEuropean Commissionen_US
bordeaux.identifier.funderIDFondation ARC pour la Recherche sur le Canceren_US
bordeaux.import.sourcedissemin
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Cell%20Biology&rft.date=2013-09&rft.volume=15&rft.issue=9&rft.spage=1107-1115&rft.epage=1107-1115&rft.eissn=1465-7392&rft.issn=1465-7392&rft.au=EZAN,%20Jerome&LASVAUX,%20Lea&GEZER,%20Aysegul&NOVAKOVIC,%20Ana&MAY-SIMERA,%20Helen&rft.genre=article


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