Stabilization of all-aqueous droplets by interfacial self-assembly of fatty acids bilayers
| dc.rights.license | open | en_US |
| hal.structure.identifier | Université de Bordeaux [UB] | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | COUDON, Noemie | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | NAVAILLES, Laurence | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | NALLET, Frederic | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | LY, Isabelle | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | BENTALEB, Ahmed | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | CHAPEL, Jean-Paul | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BEVEN, Laure | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | DOULIEZ, Jean-Paul | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | MARTIN, Nicolas | |
| dc.date.accessioned | 2022-06-10T07:00:58Z | |
| dc.date.available | 2022-06-10T07:00:58Z | |
| dc.date.issued | 2022 | |
| dc.identifier.issn | 219797 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/140178 | |
| dc.description.abstractEn | All-aqueous microdroplets produced by liquid–liquid phase separation have emerged as promising models of artificial cells, and offer new approaches for the solvent-free encapsulation of fragile solutes. Yet, the lack of a membrane on such droplets makes them intrinsically unstable against coarsening, and precludes a fine control over chemical localization, as solutes can freely diffuse through the interface. Herein, we report the construction of stable and impermeable water-in-water emulsions via the interfacial self-assembly of mixed sodium oleate/1-decanol bilayers on dextran-rich droplets produced by segregative liquid–liquid phase separation with poly(ethylene glycol). Lipids spontaneously self-assemble as multilamellar structures at the surface of the droplets as revealed by freeze-fracture transmission electron microscopy and small-angle X-ray scattering. We further demonstrate that the lipid-based membrane is impermeable to oligonucleotides and proteins, but also to a low molecular weight dye, so that a strict chemical encapsulation can be achieved by spontaneous partitioning within the droplets before membrane self-assembly. Taken together, our results highlight the ease of production of fatty acid-stabilized all-aqueous emulsions droplets able to encapsulate a range of solutes without the need of oil or organic solvents, paving the way to the construction of robust membrane-bounded, polymer-rich artificial cells. | |
| dc.description.sponsorship | Compartiments Hybrides comme Cellules Synthétiques - ANR-19-CE06-0013 | en_US |
| dc.description.sponsorship | Initiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | |
| dc.subject.en | Liquid-liquid phase separation | |
| dc.subject.en | Water-in-water emulsion | |
| dc.subject.en | Fatty acids | |
| dc.subject.en | Interfacial self-assembly | |
| dc.subject.en | Artificial cells | |
| dc.title.en | Stabilization of all-aqueous droplets by interfacial self-assembly of fatty acids bilayers | |
| dc.title.alternative | J. Colloid Interface Sci. | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.jcis.2022.02.138 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | Journal of Colloid and Interface Science | en_US |
| bordeaux.page | 257-266 | en_US |
| bordeaux.volume | 617 | en_US |
| bordeaux.hal.laboratories | Centre de Recherche Paul Pascal (CRPP) - UMR 5031 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | INRAE | |
| bordeaux.team | Colloïdes, interfaces, assemblages (CIA) | |
| bordeaux.team | Matière molle: structure et dynamique (M2SD) | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-03626236 | |
| hal.version | 1 | |
| hal.export | true | |
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