BARA, M.; MORETTO, P.; DURLACH, J.; GUIET-BARA, A.

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BARA, M.
Physiologie et physiopathologie [PP]

MORETTO, P.
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

DURLACH, J.

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Ce document a été publié dans

Magnesium Research. 2003, vol. 16, p. 35-42

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The elemental ionic distribution in the epithelial layer (EL) and in connective tissue (CT = compact layer + fibroblast layer) of the human amniotic membrane has been studied in reference samples, after conservation in a physiological fluid (Hanks' solution) and after addition of 2 mM MgCl2 in Hanks' solution. Particle induced X-ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological fluid, with regard to reference samples, the monovalent ions (Na+, K +, Cl-) concentrations were identical on both layers. This data indicates that the connective tissue, in particular the compact layer, acts as a buffer which fix minerals. Mg2+ and Ca2+ levels were higher in EL than in CT. The addition of MgCl2 in Hanks' solution induced a decrease of the monovalent ion concentrations in both layers except Na+ level in EL which remained constant, an increase of the Mg2+ level in both layers, while the Ca2+ remained constant. These data indicate the possible role of connective tissue in pregnancies complicated by poly or oligohydramnios.< Réduire

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Centre d'Études Nucléaires de Bordeaux Gradignan (CENBG)