KOSIOR, Ewelina; BOHIC, Sylvain; SUHONEN, Heikki; ORTEGA, Richard; DEVÈS, Guillaume; CARMONA, Asuncion; MARCHI, Florence; GUILLET, Jean Francois; CLOETENS, Peter

doi:10.1016/j.jsb.2011.12.005

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KOSIOR, Ewelina
European Synchrotron Radiation Facility [ESRF]

BOHIC, Sylvain
INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale

SUHONEN, Heikki
European Synchrotron Radiation Facility [ESRF]

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Journal of Structural Biology. 2012-02, vol. 177, n° 2, p. 239-47

Elsevier

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Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.< Réduire

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X-ray fluorescence

phase contrast imaging

synchrotron

trace elements

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Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.

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