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dc.rights.licenseopenen_US
dc.contributor.authorSANT’ANNA-SILVA, Ana Carolina B.
dc.contributor.authorPEREZ-VALENCIA, Juan A.
dc.contributor.authorSCIACOVELLI, Marco
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
dc.contributor.authorLALOU, Claude
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
dc.contributor.authorSARLAK, Saharnaz
dc.contributor.authorTRONCI, Laura
dc.contributor.authorNIKITOPOULOU, Efterpi
dc.contributor.authorMESZAROS, Andras T.
dc.contributor.authorFREZZA, Christian
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
dc.contributor.authorROSSIGNOL, Rodrigue
dc.contributor.authorGNAIGER, Erich
dc.contributor.authorKLOCKER, Helmut
dc.date.accessioned2021-10-26T14:38:29Z
dc.date.available2021-10-26T14:38:29Z
dc.date.issued2021-04-06
dc.identifier.issn2072-6694en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/117079
dc.description.abstractEnTumor cells display metabolic alterations when compared to non-transformed cells. These characteristics are crucial for tumor development, maintenance and survival providing energy sup-plies and molecular precursors. Anaplerosis is the property of replenishing the TCA cycle, the hub of carbon metabolism, participating in the biosynthesis of precursors for building blocks or signaling molecules. In advanced prostate cancer, an upshift of succinate-driven oxidative phosphorylation via mitochondrial Complex II was reported. Here, using untargeted metabolomics, we found succinate accumulation mainly in malignant cells and an anaplerotic effect contributing to biosynthesis, amino acid, and carbon metabolism. Succinate also stimulated oxygen consumption. Malignant prostate cells displayed higher mitochondrial affinity for succinate when compared to non-malignant prostate cells and the succinate-driven accumulation of metabolites induced expression of mitochondrial complex subunits and their activities. Moreover, extracellular succinate stimulated migration, invasion, and colony formation. Several enzymes linked to accumulated metabolites in the malignant cells were found upregulated in tumor tissue datasets, particularly NME1 and SHMT2 mRNA expression. High expression of the two genes was associated with shorter disease-free survival in prostate cancer cohorts. Moreover, in-vitro expression of both genes was enhanced in prostate cancer cells upon succinate stimulation. In conclusion, the data indicate that uptake of succinate from the tumor environment has an anaplerotic effect that enhances the malignant potential of prostate cancer cells.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enAnaplerosis
dc.subject.enCancer metabolism
dc.subject.enMitochondria
dc.subject.enProstate cancer
dc.subject.enSuccinate
dc.title.enSuccinate anaplerosis has an onco-driving potential in prostate cancer cells
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cancers13071727en_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
dc.identifier.pubmed33917317en_US
bordeaux.journalCancersen_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesMaladies Rares : Génétique et Métabolisme (MRGM) - UMR 1211en_US
bordeaux.issue7en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03404503
hal.version1
hal.date.transferred2021-10-26T14:38:35Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cancers&rft.date=2021-04-06&rft.volume=13&rft.issue=7&rft.eissn=2072-6694&rft.issn=2072-6694&rft.au=SANT%E2%80%99ANNA-SILVA,%20Ana%20Carolina%20B.&PEREZ-VALENCIA,%20Juan%20A.&SCIACOVELLI,%20Marco&LALOU,%20Claude&SARLAK,%20Saharnaz&rft.genre=article


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