Visualizing and profiling lipids in the OVLT of Fat-1 and wild type mouse brains during LPS-induced systemic inflammation using AP-SMALDI MSI
| dc.rights.license | open | en_US |
| dc.contributor.author | BREDEHOFT, Janne | |
| dc.contributor.author | BHANDARI, Dhaka Ram | |
| dc.contributor.author | PFLIEGER, Fabian Johannes | |
| dc.contributor.author | SCHULZ, Sabine | |
| dc.contributor.author | KANG, Jing X. | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | LAYE, Sophie | |
| dc.contributor.author | ROTH, Joachim | |
| dc.contributor.author | GERSTBERGER, Rüdiger | |
| dc.contributor.author | MAYER, Konstantin | |
| dc.contributor.author | SPENGLER, Bernhard | |
| dc.contributor.author | RUMMEL, Christoph | |
| dc.date.accessioned | 2021-09-22T14:24:40Z | |
| dc.date.available | 2021-09-22T14:24:40Z | |
| dc.date.issued | 2019 | |
| dc.identifier.issn | 1948-7193 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/112334 | |
| dc.description.abstractEn | Lipids, including omega-3 polyunsaturated fatty acids (n-3-PUFAs), modulate brain-intrinsic inflammation during systemic inflammation. The vascular organ of the lamina terminalis (OVLT) is a brain structure important for immune-to-brain communication. We, therefore, aimed to profile the distribution of several lipids (e.g., phosphatidyl-choline/ethanolamine, PC/PE), including n-3-PUFA-carrying lipids (esterified in phospholipids), in the OVLT during systemic lipopolysaccharide(LPS)-induced inflammation. We injected wild type and endogenously n-3-PUFA producing fat-1 transgenic mice with LPS (i.p., 2.5 mg/kg) or PBS. Brain samples were analyzed using immunohistochemistry and high-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization orbital trapping mass spectrometry imaging (AP-SMALDI-MSI) for spatial resolution of lipids. Depending on genotype and treatment, several distinct distribution patterns were observed for lipids [e.g., lyso(L)PC (16:0)/(18:0)] proposed to be involved in inflammation. The distribution patterns ranged from being homogeneously disseminated [LPC (18:1)], absent/reduced signaling within the OVLT relative to adjacent preoptic tissue [PE (38:6)], either treatment- and genotype-dependent or independent low signal intensities [LPC (18:0)], treatment- and genotype-dependent [PC 38:6)] or independent accumulation in the OVLT [PC (38:7)], and accumulation in commissures, e.g., nerve fibers like the optic nerve [LPE (18:1)]. Overall, screening of lipid distribution patterns revealed distinct inflammation-induced changes in the OVLT, highlighting the prominent role of lipid metabolism in brain inflammation. Moreover, known and novel candidates for brain inflammation and immune-to-brain communication were detected specifically within this pivotal brain structure, a window between the periphery and the brain. The biological significance of these newly identified lipids abundant in the OVLT and the adjacent preoptic area remains to be further analyzed. | |
| dc.language.iso | EN | en_US |
| dc.subject | MALDI imaging | |
| dc.subject | Vascular organ of laminae terminalis | |
| dc.subject | Lipopolysaccharide | |
| dc.subject | Immune-to-brain communication | |
| dc.subject | Brain lipids | |
| dc.subject | Omega-3 polyunsaturated fatty acids | |
| dc.title.en | Visualizing and profiling lipids in the OVLT of Fat-1 and wild type mouse brains during LPS-induced systemic inflammation using AP-SMALDI MSI | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1021/acschemneuro.9b00435 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] | en_US |
| dc.identifier.pubmed | 31513369 | en_US |
| bordeaux.journal | ACS Chemical Neuroscience | en_US |
| bordeaux.page | 4394-4406 | en_US |
| bordeaux.volume | 10 | en_US |
| bordeaux.hal.laboratories | NutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286 | en_US |
| bordeaux.issue | 10 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.team | Psychoneuroimmunologie et Nutrition: Approches expérimentales et cliniques | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Deutsche Forschungsgemeinschaft | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Chemical%20Neuroscience&rft.date=2019&rft.volume=10&rft.issue=10&rft.spage=4394-4406&rft.epage=4394-4406&rft.eissn=1948-7193&rft.issn=1948-7193&rft.au=BREDEHOFT,%20Janne&BHANDARI,%20Dhaka%20Ram&PFLIEGER,%20Fabian%20Johannes&SCHULZ,%20Sabine&KANG,%20Jing%20X.&rft.genre=article |
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