Tetracosahexaenoylethanolamide, a novel N-acylethanolamide, is elevated in ischemia and increases neuronal output
| dc.rights.license | open | en_US |
| hal.structure.identifier | University of Toronto | |
| dc.contributor.author | LIN, Lin | |
| hal.structure.identifier | University of Toronto | |
| dc.contributor.author | METHEREL, Adam | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | DI MICELI, Mathieu | |
| hal.structure.identifier | University of Toronto | |
| dc.contributor.author | LIU, Zhen | |
| hal.structure.identifier | University of Toronto | |
| dc.contributor.author | SAHIN, Cigdem | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | FIORAMONTI, Xavier | |
| hal.structure.identifier | University of Toronto | |
| dc.contributor.author | CUMMINS, Carolyn | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | LAYE, Sophie
ORCID: 0000-0002-3843-1012 IDREF: 11366883X | |
| hal.structure.identifier | University of Toronto | |
| dc.contributor.author | BAZINET, Richard | |
| dc.date.accessioned | 2021-09-13T09:08:19Z | |
| dc.date.available | 2021-09-13T09:08:19Z | |
| dc.date.issued | 2020-11 | |
| dc.identifier.issn | 0022-2275 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/112207 | |
| dc.description.abstractEn | N-acylethanolamines (NAEs) are endogenous lipid-signaling molecules derived from fatty acids that regulate numerous biological functions, including in the brain. Interestingly, NAEs are elevated in the absence of fatty acid amide hydrolase (FAAH) and following CO2-induced ischemia/hypercapnia, suggesting a neuroprotective response. Tetracosahexaenoic acid (THA) is a product and precursor to DHA; however, the NAE product, tetracosahexaenoylethanolamide (THEA), has never been reported. Presently, THEA was chemically synthesized as an authentic standard to confirm THEA presence in biological tissues. Whole brains were collected and analyzed for unesterified THA, total THA, and THEA in wild-type and FAAH-KO mice that were euthanized by either head-focused microwave fixation, CO2 + microwave, or CO2 only. PPAR activity by transient transfection assay and ex vivo neuronal output in medium spiny neurons (MSNs) of the nucleus accumbens by patch clamp electrophysiology were determined following THEA exposure. THEA in the wild-type mice was nearly doubled (P 0.05) transcriptional activity of PPARs relative to control, but 100 nM of THEA increased (P < 0.001) neuronal output in MSNs of the nucleus accumbens. Here were identify a novel NAE, THEA, in the brain that is elevated upon ischemia/hypercapnia and by KO of the FAAH enzyme. While THEA did not activate PPAR, it augmented the excitability of MSNs in the nucleus accumbens. Overall, our results suggest that THEA is a novel NAE that is produced in the brain upon ischemia/hypercapnia and regulates neuronal excitation. | |
| dc.language.iso | EN | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | tetracosahexaenoic acid | |
| dc.subject.en | fatty acid amide hydrolase | |
| dc.subject.en | patch clamp | |
| dc.subject.en | neurons | |
| dc.subject.en | brain lipids | |
| dc.subject.en | fatty acid | |
| dc.subject.en | metabolism | |
| dc.subject.en | peroxisome proliferator-activated receptors | |
| dc.subject.en | fatty acid amide hydrolase-knockout | |
| dc.title.en | Tetracosahexaenoylethanolamide, a novel N-acylethanolamide, is elevated in ischemia and increases neuronal output | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1194/jlr.RA120001024 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio] | en_US |
| bordeaux.journal | Journal of Lipid Research | en_US |
| bordeaux.page | 1480-1490 | en_US |
| bordeaux.volume | 61 | en_US |
| bordeaux.hal.laboratories | NutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286 | en_US |
| bordeaux.issue | 11 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.team | Psychoneuroimmunologie et Nutrition: Approches expérimentales et cliniques | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-03167174 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Lipid%20Research&rft.date=2020-11&rft.volume=61&rft.issue=11&rft.spage=1480-1490&rft.epage=1480-1490&rft.eissn=0022-2275&rft.issn=0022-2275&rft.au=LIN,%20Lin&METHEREL,%20Adam&DI%20MICELI,%20Mathieu&LIU,%20Zhen&SAHIN,%20Cigdem&rft.genre=article |
