Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures
| dc.rights.license | open | en_US |
| dc.contributor.author | FERNANDEZ, Roberto | |
| dc.contributor.author | GARATE, Jone | |
| dc.contributor.author | TOLENTINO-CORTEZ, Tarson | |
| dc.contributor.author | HERRAIZ, Ainara | |
| dc.contributor.author | LOMBARDERO, Laura | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | DUCROCQ, Fabien | |
| dc.contributor.author | RODRIGUEZ-PUERTAS, Rafael | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | TRIFILIEFF, Pierre | |
| dc.contributor.author | ASTIGARRAGA, Egoitz | |
| dc.contributor.author | BARREDA-GOMEZ, Gabriel | |
| dc.contributor.author | FERNANDEZ, Jose A. | |
| dc.date.accessioned | 2021-08-31T10:11:18Z | |
| dc.date.available | 2021-08-31T10:11:18Z | |
| dc.date.issued | 2019-12-17 | |
| dc.identifier.issn | 1520-6882 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/110265 | |
| dc.description.abstractEn | The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell's homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with proteins. Here, we present a new methodology that combines microarrays with mass spectrometry to obtain the lipid fingerprint of samples of a different nature in a standardized and fast way, with minimal sample consumption. As a proof of concept, we use the methodology to obtain the lipid fingerprint of 20 rat tissues and to create a lipid library for tissue classification. Then, we combine those results with immunohistochemistry and enzymatic assays to unveil the relationship between some lipid species and two enzymes. Finally, we demonstrate the performance of the methodology to explore changes in lipid composition of the nucleus accumbens from mice subjected to two lipid diets. | |
| dc.description.sponsorship | Impact de la composition lipidique membranaire sur la transmission dopaminergique dépendante du récepteur D2 et la motivation | en_US |
| dc.language.iso | EN | en_US |
| dc.subject.en | Algorithms | |
| dc.subject.en | Lipids | |
| dc.subject.en | Microarrays | |
| dc.subject.en | Membranes | |
| dc.subject.en | Rodent models | |
| dc.title.en | Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1021/acs.analchem.9b04529 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] | en_US |
| dc.identifier.pubmed | 31751120 | en_US |
| bordeaux.journal | Analytical Chemistry | en_US |
| bordeaux.page | 15967-15973 | en_US |
| bordeaux.volume | 91 | en_US |
| bordeaux.hal.laboratories | NutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286 | en_US |
| bordeaux.issue | 24 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.team | Psychoneuroimmunologie et Nutrition: Approches expérimentales et cliniques | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Université de Bordeaux | en_US |
| bordeaux.identifier.funderID | Institut National de la Recherche Agronomique | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20Chemistry&rft.date=2019-12-17&rft.volume=91&rft.issue=24&rft.spage=15967-15973&rft.epage=15967-15973&rft.eissn=1520-6882&rft.issn=1520-6882&rft.au=FERNANDEZ,%20Roberto&GARATE,%20Jone&TOLENTINO-CORTEZ,%20Tarson&HERRAIZ,%20Ainara&LOMBARDERO,%20Laura&rft.genre=article |
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