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dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPEDROSA, C. R.
dc.contributor.authorARL, D.
dc.contributor.authorGRYSAN, P.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorKHAN, I.
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorDURRIEU, S.
dc.contributor.authorKRISHNAMOORTHY, S.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie‐Christine
dc.date.accessioned2021-07-16T14:32:14Z
dc.date.available2021-07-16T14:32:14Z
dc.date.issued2019
dc.identifier.issn1944-8244en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/109298
dc.description.abstractEnNanotopography with length scales of the order of extracellular matrix elements offers the possibility of regulating cell behavior. Investigation of the impact of nanotopography on cell response has been limited by the inability to precisely control geometries, especially at high spatial resolutions and across practically large areas. In this paper, we demonstrate well-controlled and periodic nanopillar arrays of silicon and investigate their impact on osteogenic differentiation of human mesenchymal stem cells (hMSCs). Silicon nanopillar arrays with critical dimensions in the range of 40-200 nm, exhibiting standard deviations below 15% across full wafers, were realized using the self-assembly of block copolymer colloids. Immunofluorescence and quantitative polymerase chain reaction measurements reveal clear dependence of osteogenic differentiation of hMSCs on the diameter and periodicity of the arrays. Further, the differentiation of hMSCs was found to be dependent on the age of the donor. While osteoblastic differentiation was found to be promoted by the pillars with larger diameters and heights independent of donor age, they were found to be different for different spacings. Pillar arrays with smaller pitch promoted differentiation from a young donor, while a larger spacing promoted those of an old donor. These findings can contribute for the development of personalized treatments of bone diseases, namely, novel implant nanostructuring depending on patient age.
dc.language.isoENen_US
dc.subject.enOsteogenic differentiation
dc.subject.enCells
dc.subject.enSilicon
dc.subject.enGenetics
dc.subject.enDifferentiation
dc.title.enControlled Nanoscale Topographies for Osteogenic Differentiation of Mesenchymal Stem Cells
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acsami.8b21393en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalACS Applied Materials & Interfacesen_US
bordeaux.page8858-8866en_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.issue9en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02157703
hal.exportfalse
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