Show simple item record

dc.rights.licenseopenen_US
dc.contributor.authorLIU, L. W.
dc.contributor.authorVIEL, A.
dc.contributor.authorLE SAUX, G.
dc.contributor.authorPLAWINSKI, Laurent
dc.contributor.authorMUGGIOLU, G.
dc.contributor.authorBARBERET, Philippe
IDREF: 078137748
dc.contributor.authorPEREIRA, M.
dc.contributor.authorAYELA, Cedric
IDREF: 125917287
dc.contributor.authorSEZNEC, Herve
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie‐Christine
dc.contributor.authorOLIVE, J. M.
dc.contributor.authorAUDOIN, B.
dc.date.accessioned2021-07-16T09:49:05Z
dc.date.available2021-07-16T09:49:05Z
dc.date.issued2019
dc.identifier.issn2045-2322en_US
dc.identifier.otherhttps://doi.org/10.1038/s41598-019-42718-5en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/109276
dc.description.abstractEnCell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited by optics and an out-of-plane accuracy down to a tenth of the optical wavelength. For this, GHz coherent acoustic phonons and their resonance harmonics were tracked by means of an ultrafast opto-acoustic technique. After illustrating the measurement accuracy with cell-mimetic polymer films we map the 3D morphology of an entire osteosarcoma cell. The resulting image complies with the image obtained by standard atomic force microscopy, and both reveal very close roughness mean values. In addition, while scanning macrophages and monocytes, we demonstrate an enhanced contrast of thickness mapping by taking advantage of the detection of high-frequency resonance harmonics. Illustrations are given with the remote quantitative imaging of the nucleus thickness gradient of migrating monocyte cells.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.title.enRemote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41598-019-42718-5en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalScientific Reportsen_US
bordeaux.volume9en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02123347
hal.version1
hal.date.transferred2021-07-16T09:49:12Z
hal.exporttrue
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2019&rft.volume=9&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=LIU,%20L.%20W.&VIEL,%20A.&LE%20SAUX,%20G.&PLAWINSKI,%20Laurent&MUGGIOLU,%20G.&rft.genre=article


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record