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Label-free multi-parametric imaging of single cells: dual picosecond optoacoustic microscopy
| dc.rights.license | open | en_US |
| dc.contributor.author | LIU, Liwang | |
| dc.contributor.author | PLAWINSKI, Laurent | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | DURRIEU, Marie-Christine | |
| dc.contributor.author | AUDOIN, Bertrand
IDREF: 077252918 | |
| dc.date.accessioned | 2021-07-16T09:47:26Z | |
| dc.date.available | 2021-07-16T09:47:26Z | |
| dc.date.issued | 2019 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/109275 | |
| dc.description.abstractEn | Advances in microscopy with new visualization possibilities often bring dramatic progress to our understanding of the intriguing cellular machinery. Picosecond optoacoustic micro-spectroscopy is an optical technique based on ultrafast pump-probe generation and detection of hypersound on time durations of picoseconds and length scales of nanometers. It is experiencing a renaissance as a versatile imaging tool for cell biology research after a plethora of applications in solid-state physics. In this emerging context, this work reports on a dual-probe architecture to carry out real-time parallel detection of the hypersound propagation inside a cell that is cultured on a metallic substrate, and of the hypersound reflection at the metal/cell adhesion interface. Using this optoacoustic modality, several biophysical properties of the cell can be measured in a noncontact and label-free manner. Its abilities are demonstrated with the multiple imaging of a mitotic macrophage-like cell in a single run experiment. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | cell adhesion | |
| dc.subject.en | cell mechanics | |
| dc.subject.en | mitosis and division | |
| dc.subject.en | picosecond optoacoustics | |
| dc.subject.en | single-cell imaging | |
| dc.title.en | Label-free multi-parametric imaging of single cells: dual picosecond optoacoustic microscopy | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1002/jbio.201900045 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | Journal of Biophotonics | en_US |
| bordeaux.page | e201900045-e201900045 | en_US |
| bordeaux.volume | 12 | en_US |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | en_US |
| bordeaux.issue | 8 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-03160465 | |
| hal.export | false | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biophotonics&rft.date=2019&rft.volume=12&rft.issue=8&rft.spage=e201900045-e201900045&rft.epage=e201900045-e201900045&rft.au=LIU,%20Liwang&PLAWINSKI,%20Laurent&DURRIEU,%20Marie-Christine&AUDOIN,%20Bertrand&rft.genre=article |
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