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Dermal CD14(+) Dendritic Cell and Macrophage Infection by Dengue Virus Is Stimulated by Interleukin-4

dc.contributor.authorSCHAEFFER, Evelyne
dc.contributor.authorFLACHER, Vincent
dc.contributor.authorPAPAGEORGIOU, Vasiliki
dc.contributor.authorDECOSSAS, Marion
dc.contributor.authorFAUNY, Jean-Daniel
dc.contributor.authorKRAEMER, Melanie
dc.contributor.authorMUELLER, Christopher G.
dc.date.accessioned2020-09-03T08:01:54Z
dc.date.available2020-09-03T08:01:54Z
dc.date.issued2015
dc.identifier.issn0022-202X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10925
dc.description.abstractEnDengue virus (DENV) is responsible for the most prevalent arthropod-borne viral infection in humans. Events decisive for disease development occur in the skin after virus inoculation by the mosquito. Yet, the role of human dermis-resident immune cells in dengue infection and disease remains elusive. Here we investigated how dermal dendritic cells (dDCs) and macrophages (dMs) react to DENV and impact on immunopathology. We show that both CD1c(+) and CD14(+) dDC subsets were infected, but viral load greatly increased in CD14(+) dDCs upon IL-4 stimulation, which correlated with upregulation of virus-binding lectins Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Nonintegrin (DC-SIGN/CD209) and mannose receptor (CD206). IL-4 also enhanced T-cell activation by dDCs, which was further increased upon dengue infection. dMs purified from digested dermis were initially poorly infected but actively replicated the virus and produced TNF-alpha upon lectin upregulation in response to IL-4. DC-SIGN(+) cells are abundant in inflammatory skin with scabies infection or Th2-type dermatitis, suggesting that skin reactions to mosquito bites heighten the risk of infection and subsequent immunopathology. Our data identify dDCs and dMs as primary arbovirus target cells in humans and suggest that dDCs initiate a potent virus-directed T-cell response, whereas dMs fuel the inflammatory cascade characteristic of dengue fever.
dc.language.isoen
dc.title.enDermal CD14(+) Dendritic Cell and Macrophage Infection by Dengue Virus Is Stimulated by Interleukin-4
dc.typeArticle de revue
dc.identifier.doi10.1038/jid.2014.525
dc.subject.halChimie/Matériaux
bordeaux.journalJOURNAL OF INVESTIGATIVE DERMATOLOGY
bordeaux.page1743-1751
bordeaux.volume135
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue7
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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