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Isolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection

dc.contributor.authorSLIMENE, Imen Ben
dc.contributor.authorTABBENE, Olfa
dc.contributor.authorGHARBI, Dorra
dc.contributor.authorMNASRI, Bacem
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSCHMITTER, Jean Marie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorURDACI, Maria C.
ORCID: 0000-0002-0868-0050
IDREF: 033307679
dc.contributor.authorLIMAM, Ferid
dc.date.accessioned2020-09-03T08:01:52Z
dc.date.available2020-09-03T08:01:52Z
dc.date.issued2015
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10917
dc.description.abstractEnAmong nine chitinase-producing strains isolated from Tunisian soil, one isolate called S213 exhibited a potent chitinolytic activity. S213 strain was identified as Bacillus licheniformis by API 50CH system and sequence analysis of its partial 16S ribosomal DNA. Chitinolytic activity was induced either by colloidal chitin or fungal cell walls, and the highest chitinase activity reached at the late stationary phase exhibiting optimal temperature and pH of 50-60°C and pH6.0, respectively. SDS-PAGE analysis of the secreted colloidal chitin-induced proteins showed a major protein of about 65kDa. This protein was identified as chitinase on the basis of its peptide sequences which displayed high homology with chitinase sequence of B. licheniformis ATCC 14580. Moreover, chitinolytic activity containing supernatant inhibited the growth of several phytopathogenic fungi including Phoma medicaginis. Interestingly, S213 strain reduced efficiently the damping-off disease caused by P. medicaginis in Medicago truncatula and should be envisaged in enzyme-based biopesticides against phytopathogen application.
dc.language.isoen
dc.title.enIsolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection
dc.typeArticle de revue
dc.identifier.doi10.1007/s12010-015-1520-7
dc.subject.halChimie/Matériaux
bordeaux.journalApplied biochemistry and biotechnology
bordeaux.page3494-506
bordeaux.volume175
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue7
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applied%20biochemistry%20and%20biotechnology&rft.date=2015&rft.volume=175&rft.issue=7&rft.spage=3494-506&rft.epage=3494-506&rft.au=SLIMENE,%20Imen%20Ben&TABBENE,%20Olfa&GHARBI,%20Dorra&MNASRI,%20Bacem&SCHMITTER,%20Jean%20Marie&rft.genre=article


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