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Detection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions

dc.contributor.authorROCA, E.
dc.contributor.authorLACROIX, R.
dc.contributor.authorJUDICONE, C.
dc.contributor.authorLAROUMAGNE, S.
dc.contributor.authorROBERT, S.
dc.contributor.authorCOINTE, S.
dc.contributor.authorMULLER, A.
dc.contributor.authorKASPI, E.
dc.contributor.authorROLL, P.
dc.contributor.authorBRISSON, Alain
dc.contributor.authorTANTUCCI, C.
dc.contributor.authorASTOUL, P.
dc.contributor.authorDIGNAT-GEORGE, F.
dc.date.accessioned2020-09-03T07:56:08Z
dc.date.available2020-09-03T07:56:08Z
dc.date.issued2016
dc.identifier.issn1949-2553
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10826
dc.description.abstractEnPleural biomarkers allowing to mini-invasively discriminate benign from malignant pleural effusions are needed. Among potential candidates, microparticles (MPs) are extracellular vesicles that vectorize antigen derived from the parent cell. We hypothesized that tumor-derived MPs could be present in the pleural liquid and help to identify patients with malignant pleural effusions. Using highly sensitive flow cytometry and cryo-electron microscopy, we showed that large amounts of MPs from hematopoietic and vascular origin could be detectable in pleural fluids. Their level did not differ between benign (n = 14) and malignant (n = 71) pleural effusions. Analysis of selected tumoral associated antigens (podoplanin, mucin 1 and EpCAM, epithelial-cell-adhesion-molecule) evidenced for the first time the presence of tumor-derived MPs expressing EpCAM in malignant pleural fluids only (Specificity = 93%, Sensitivity = 49% and 45% for flow cytometry and ELISA, respectively). The detection of EpCAM-positive-MPs (EpCAM + MPs) by flow cytometry showed a better specificity and sensitivity than ELISA to distinguish between pleural carcinoma and the others malignant pleural effusions (MPE; Sp: 96% vs 89%; Se: 79% vs 66%). Combining EpCAM+ MPs and cytology improved the diagnosis of MPE compared to cytology alone. This study establishes the basis for using EpCAM+ MPs as a promising new biomarker that could be added to the armamentarium to mini-invasively identify patients with malignant pleural effusions.
dc.language.isoen
dc.title.enDetection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions
dc.title.alternativeOncotarget
dc.typeArticle de revue
dc.identifier.doi10.18632/oncotarget.6581
dc.subject.halChimie/Matériaux
bordeaux.journalOncotarget
bordeaux.page3357-66
bordeaux.volume7
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue3
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Oncotarget&rft.date=2016&rft.volume=7&rft.issue=3&rft.spage=3357-66&rft.epage=3357-66&rft.eissn=1949-2553&rft.issn=1949-2553&rft.au=ROCA,%20E.&LACROIX,%20R.&JUDICONE,%20C.&LAROUMAGNE,%20S.&ROBERT,%20S.&rft.genre=article


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