Detection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions
| dc.contributor.author | ROCA, E. | |
| dc.contributor.author | LACROIX, R. | |
| dc.contributor.author | JUDICONE, C. | |
| dc.contributor.author | LAROUMAGNE, S. | |
| dc.contributor.author | ROBERT, S. | |
| dc.contributor.author | COINTE, S. | |
| dc.contributor.author | MULLER, A. | |
| dc.contributor.author | KASPI, E. | |
| dc.contributor.author | ROLL, P. | |
| dc.contributor.author | BRISSON, Alain | |
| dc.contributor.author | TANTUCCI, C. | |
| dc.contributor.author | ASTOUL, P. | |
| dc.contributor.author | DIGNAT-GEORGE, F. | |
| dc.date.accessioned | 2020-09-03T07:56:08Z | |
| dc.date.available | 2020-09-03T07:56:08Z | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 1949-2553 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10826 | |
| dc.description.abstractEn | Pleural biomarkers allowing to mini-invasively discriminate benign from malignant pleural effusions are needed. Among potential candidates, microparticles (MPs) are extracellular vesicles that vectorize antigen derived from the parent cell. We hypothesized that tumor-derived MPs could be present in the pleural liquid and help to identify patients with malignant pleural effusions. Using highly sensitive flow cytometry and cryo-electron microscopy, we showed that large amounts of MPs from hematopoietic and vascular origin could be detectable in pleural fluids. Their level did not differ between benign (n = 14) and malignant (n = 71) pleural effusions. Analysis of selected tumoral associated antigens (podoplanin, mucin 1 and EpCAM, epithelial-cell-adhesion-molecule) evidenced for the first time the presence of tumor-derived MPs expressing EpCAM in malignant pleural fluids only (Specificity = 93%, Sensitivity = 49% and 45% for flow cytometry and ELISA, respectively). The detection of EpCAM-positive-MPs (EpCAM + MPs) by flow cytometry showed a better specificity and sensitivity than ELISA to distinguish between pleural carcinoma and the others malignant pleural effusions (MPE; Sp: 96% vs 89%; Se: 79% vs 66%). Combining EpCAM+ MPs and cytology improved the diagnosis of MPE compared to cytology alone. This study establishes the basis for using EpCAM+ MPs as a promising new biomarker that could be added to the armamentarium to mini-invasively identify patients with malignant pleural effusions. | |
| dc.language.iso | en | |
| dc.title.en | Detection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions | |
| dc.title.alternative | Oncotarget | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.18632/oncotarget.6581 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.journal | Oncotarget | |
| bordeaux.page | 3357-66 | |
| bordeaux.volume | 7 | |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
| bordeaux.issue | 3 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Oncotarget&rft.date=2016&rft.volume=7&rft.issue=3&rft.spage=3357-66&rft.epage=3357-66&rft.eissn=1949-2553&rft.issn=1949-2553&rft.au=ROCA,%20E.&LACROIX,%20R.&JUDICONE,%20C.&LAROUMAGNE,%20S.&ROBERT,%20S.&rft.genre=article |
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