NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils
dc.contributor.author | STANEK, Jan | |
dc.contributor.author | ANDREAS, Loren B. | |
dc.contributor.author | JAUDZEMS, Kristaps | |
dc.contributor.author | CALA, Diane | |
dc.contributor.author | LALLI, Daniela | |
dc.contributor.author | BERTARELLO, Andrea | |
dc.contributor.author | SCHUBEIS, Tobias | |
dc.contributor.author | AKOPJANA, Inara | |
dc.contributor.author | KOTELOVICA, Svetlana | |
dc.contributor.author | TARS, Kaspars | |
dc.contributor.author | PICA, Andrea | |
dc.contributor.author | LEONE, Serena | |
dc.contributor.author | PICONE, Delia | |
dc.contributor.author | XU, Zhi-Qiang | |
dc.contributor.author | DIXON, Nicholas E. | |
dc.contributor.author | MARTINEZ, Denis | |
dc.contributor.author | BERBON, Melanie | |
dc.contributor.author | EL MAMMERI, Nadia | |
dc.contributor.author | NOUBHANI, Abdelmajid | |
dc.contributor.author | SAUPE, Sven | |
dc.contributor.author | HABENSTEIN, Birgit | |
dc.contributor.author | LOQUET, Antoine | |
dc.contributor.author | PINTACUDA, Guido | |
dc.date.accessioned | 2020-09-03T07:56:06Z | |
dc.date.available | 2020-09-03T07:56:06Z | |
dc.date.issued | 2016 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10821 | |
dc.description.abstractEn | We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid-state NMR spectroscopy with 100-111 kHz magic-angle spinning (MAS). The excellent resolution in the Calpha-Halpha plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, a capsid and amyloid fibrils. A set of 3D spectra based on a Calpha-Halpha detection block was developed and applied for the sequence-specific backbone and aliphatic side-chain resonance assignment using only 500 mug of sample. These developments accelerate structural studies of biomolecular assemblies available in submilligram quantities without the need of protein deuteration. | |
dc.language.iso | en | |
dc.title.en | NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1002/anie.201607084 | |
dc.subject.hal | Chimie/Matériaux | |
bordeaux.journal | Angewandte Chemie (International ed. in English) | |
bordeaux.page | 15503-15509 | |
bordeaux.volume | 55 | |
bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
bordeaux.issue | 50 | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | Bordeaux INP | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angewandte%20Chemie%20(International%20ed.%20in%20English)&rft.date=2016&rft.volume=55&rft.issue=50&rft.spage=15503-15509&rft.epage=15503-15509&rft.au=STANEK,%20Jan&ANDREAS,%20Loren%20B.&JAUDZEMS,%20Kristaps&CALA,%20Diane&LALLI,%20Daniela&rft.genre=article |
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