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Tracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope

dc.contributor.authorSTREPPA, L.
dc.contributor.authorBERGUIGA, L.
dc.contributor.authorPROVERA, E. B.
dc.contributor.authorRATTI, F.
dc.contributor.authorGOILLOT, E.
dc.contributor.authorTORRES, C. M.
dc.contributor.authorSCHAEFFER, L.
dc.contributor.authorELEZGARAY, Juan
dc.contributor.authorARNEODO, A.
dc.contributor.authorARGOUL, F.
dc.date.accessioned2020-09-03T07:56:06Z
dc.date.available2020-09-03T07:56:06Z
dc.date.issued2016
dc.identifier.isbn0277-786X 978-1-62841-958-0
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10820
dc.description.abstractEnWe introduce a high resolution scanning surface plasmon microscope for long term imaging of living adherent mouse myoblast cells. The coupling of a high numerical aperture objective lens with a fibered heterodyne interferometer provides both enhanced sensitivity and long term stability. This microscope takes advantage of the plasmon resonance excitation and the amplification of the electromagnetic field in near -field distance to the gold coated coverslip. This plasmon enhanced evanescent wave microscopy is particularly attractive for the study of cell adhesion and motility since it can be operated without staining of the biological sample. We show that this microscope allows very long-term imaging of living samples, and that it can capture and follow the temporal deformation of C2C12 myoblast cell protusions (lamellipodia), during their migration on a flat surface.
dc.language.isoen
dc.title.enTracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope
dc.typeChapitre d'ouvrage
dc.identifier.doi10.1117/12.2211331
dc.subject.halChimie/Matériaux
bordeaux.journalPlasmonics in Biology and Medicine Xiii
bordeaux.volume9724
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plasmonics%20in%20Biology%20and%20Medicine%20Xiii&rft.date=2016&rft.volume=9724&rft.au=STREPPA,%20L.&BERGUIGA,%20L.&PROVERA,%20E.%20B.&RATTI,%20F.&GOILLOT,%20E.&rft.isbn=0277-786X%20978-1-62841-958-0&rft.genre=unknown


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