Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | MAMODE CASSIM, Adiilah | |
| dc.contributor.author | NAVON, Yotam | |
| dc.contributor.author | GAO, Yu | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | DECOSSAS, Marion | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | FOUILLEN, Laetitia | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | GRELARD, Axelle | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | NAGANO, Minoru | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | LAMBERT, Olivier | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BAHAMMOU, Delphine | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | VAN DELFT, Pierre | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | MANETA-PEYRET, Lilly | |
| dc.contributor.author | SIMON-PLAS, Francoise | |
| dc.contributor.author | HEUX, Laurent | |
| dc.contributor.author | JEAN, Bruno | |
| dc.contributor.author | FRAGNETO, Giovanna | |
| dc.contributor.author | MORTIMER, Jenny C. | |
| dc.contributor.author | DELEU, Magali | |
| dc.contributor.author | LINS, Laurence | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | MONGRAND, Sébastien | |
| dc.date.accessioned | 2021-07-09T15:05:55Z | |
| dc.date.available | 2021-07-09T15:05:55Z | |
| dc.date.issued | 2021 | |
| dc.identifier.issn | 1083-351X | en_US |
| dc.identifier.other | https://www.jbc.org/action/showPdf?pii=S0021-9258%2821%2900382-3#%FE%FF%00a%00p%00p%00s%00e%00c%001 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/106505 | |
| dc.description.abstractEn | The plant plasma membrane (PM) is an essential barrier between the cell and the external environment, controlling signal perception and transmission. It consists of an asymmetrical lipid bilayer made up of three different lipid classes: sphingolipids, sterols and phospholipids. The Glycosyl Inositol Phosphoryl Ceramides (GIPCs), representing up to 40% of total sphingolipids, are assumed to be almost exclusively in the outer leaflet of the PM. However, their biological role and properties are poorly defined. In this study, we investigated the role of GIPCs in membrane organization. Since GIPCs are not commercially available, we developed a protocol to extract and isolate GIPC-enriched fractions from eudicots (cauliflower and tobacco) and monocots (leek and rice). Lipidomic analysis confirmed the presence of trihydroxylated long chain bases and 2-hydroxylated very long chain fatty acids up to 26 carbon atoms. The glycan head groups of the GIPCs from monocots and dicots were analyzed by GC-MS, revealing different sugar moieties. Multiple biophysics tools, namely Langmuir monolayer, zeta-Potential, light scattering, neutron reflectivity, solid state 2H-NMR and molecular modelling, were used to investigate the physical properties of the GIPCs, as well as their interaction with free and conjugated phytosterols. We showed that GIPCs increase the thickness and electronegativity of model membranes, interact differentially with the different phytosterols species and regulate the gel-to-fluid phase transition during temperature variations. These results unveil the multiple roles played by GIPCs in the plant plasma membrane. | |
| dc.description.sponsorship | Vers un modèle intégratif de la bicouche lipidique de la membrane plasmique végétale | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Cryo-EM | |
| dc.subject.en | Solid state NMR | |
| dc.subject.en | Neutron reflectivity | |
| dc.subject.en | Langmuir monolayer | |
| dc.subject.en | ζ-Potential | |
| dc.subject.en | Modelling | |
| dc.subject.en | Plant | |
| dc.subject.en | Plasma membrane | |
| dc.subject.en | Sphingolipids | |
| dc.subject.en | GIPC | |
| dc.subject.en | Phytosterol | |
| dc.subject.en | Purification | |
| dc.subject.en | Lipidomics | |
| dc.title.en | Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.jbc.2021.100602 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | The Journal of biological chemistry | en_US |
| bordeaux.page | 100602-100602 | en_US |
| bordeaux.volume | 296 | en_US |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-03189252 | |
| hal.export | false | |
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