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A Two-Component Adhesive: Tau Fibrils Arise from a Combination of a Well-Defined Motif and Conformationally Flexible Interactions

dc.contributor.authorXIANG, Shengqi
dc.contributor.authorKULMINSKAYA, Natalia
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorHABENSTEIN, Birgit
dc.contributor.authorBIERNAT, Jacek
dc.contributor.authorTEPPER, Katharina
dc.contributor.authorPAULAT, Maria
dc.contributor.authorGRIESINGER, Christian
dc.contributor.authorBECKER, Stefan
dc.contributor.authorLANGE, Adam
dc.contributor.authorMANDELKOW, Eckhard
dc.contributor.authorLINSER, Rasmus
dc.date.accessioned2020-07-08T08:44:04Z
dc.date.available2020-07-08T08:44:04Z
dc.date.issued2017-02-22
dc.identifier.issn0002-7863
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10184
dc.description.abstractEnFibrillar aggregates of A beta and Tau in the brain are the major hallmarks of Alzheimer's disease. Most Tau fibers have a twisted appearance, but the twist can be variable and even absent. This ambiguity, which has also been associated with different phenotypes of tauopathies, has led to controversial assumptions about fibril constitution, and it is unclear to-date what the molecular causes of this polymorphism are. To tackle this question, we used solid-state NMR strategies providing assignments of non-seeded three-repeat-domain Tau(3RD) with an inherent heterogeneity. This is in contrast to the general approach to characterize the most homogeneous preparations by construct truncation or intricate seeding protocols. Here, carbon and nitrogen chemical-shift conservation between fibrils revealed invariable secondary-structure properties, however, with inter-monomer interactions variable among samples. Residues with variable amide shifts are localized mostly to N- and C-terminal regions within the rigid beta structure in the repeat region of Tau(3RD). By contrast, the hexapeptide motif in repeat R3, a crucial motif for fibril formation, shows strikingly low variability of all NMR parameters: Starting as a nucleation site for monomer monomer contacts, this six-residue sequence element also turns into a well-defined structural element upon fibril formation. Given the absence of external causes in vitro, the interplay of structurally differently conserved elements in this protein likely reflects an intrinsic property of Tau fibrils.
dc.title.enA Two-Component Adhesive: Tau Fibrils Arise from a Combination of a Well-Defined Motif and Conformationally Flexible Interactions
dc.typeArticle de revue
dc.identifier.doi10.1021/jacs.6b09619
dc.subject.halChimie/Matériaux
bordeaux.journalJournal of the American Chemical Society
bordeaux.page2639-2646
bordeaux.volume139
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue7
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20the%20American%20Chemical%20Society&rft.date=2017-02-22&rft.volume=139&rft.issue=7&rft.spage=2639-2646&rft.epage=2639-2646&rft.eissn=0002-7863&rft.issn=0002-7863&rft.au=XIANG,%20Shengqi&KULMINSKAYA,%20Natalia&HABENSTEIN,%20Birgit&BIERNAT,%20Jacek&TEPPER,%20Katharina&rft.genre=article


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