Avis

Ceci n'est pas la version la plus récente de cet item. On peut trouver la version la plus récente à : https://oskar-bordeaux.fr/handle/20.500.12278/79129.2

Afficher la notice abrégée

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties.

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRÉMY, Murielle
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFERRARO, Francesca
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLE SALVER, Pierre
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, Sylvie
hal.structure.identifierCentre de Recherche Cardio-thoracique de Bordeaux, U1045
dc.contributor.authorGENOT, Elisabeth
dc.contributor.authorDJAVAHERI-MERGNY, Mojgan
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHÉBAUD, Noélie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOIZIAU, Claudine
ORCID: 0000-0002-0475-2571
IDREF: 85228370
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOEUF, Hélène
ORCID: 0000-0002-3006-8773
IDREF: 03205453X
dc.date.accessioned2021-06-14T14:59:56Z
dc.date.available2021-06-14T14:59:56Z
dc.date.issued2019-11-21
dc.identifier.issn2073-4409en_US
dc.identifier.otherhttps://www.mdpi.com/2073-4409/8/12/1485/s1en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79129
dc.description.abstractEnStem cells isolated from the apical papilla of wisdom teeth (SCAPs) are an attractive model for tissue repair due to their availability, high proliferation rate and potential to differentiate in vitro towards mesodermal and neurogenic lineages. Adult stem cells, such as SCAPs, develop in stem cell niches in which the oxygen concentration [O] is low (3-8% compared with 21% of ambient air). In this work, we evaluate the impact of low [O] on the physiology of SCAPs isolated and processed in parallel at 21% or 3% O without any hyperoxic shock in ambient air during the experiment performed at 3% O. We demonstrate that SCAPs display a higher proliferation capacity at 3% O than in ambient air with elevated expression levels of two cell surface antigens: the alpha-6 integrin subunit (CD49f) and the embryonic stem cell marker (SSEA4). We show that the mesodermal differentiation potential of SCAPs is conserved at early passage in both [O], but is partly lost at late passage and low [O], conditions in which SCAPs proliferate efficiently without any sign of apoptosis. Unexpectedly, we show that autophagic flux is active in SCAPs irrespective of [O] and that this process remains high in cells even after prolonged exposure to 3% O.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enAutophagy
dc.subject.enCell Culture Techniques
dc.subject.enCell Differentiation
dc.subject.enCell Hypoxia
dc.subject.enCell Proliferation
dc.subject.enCells
dc.subject.enCultured
dc.subject.enDental Papilla
dc.subject.enHumans
dc.subject.enIntegrin alpha6
dc.subject.enMembrane Proteins
dc.subject.enMolar
dc.subject.enThird
dc.subject.enOsteogenesis
dc.subject.enOxygen
dc.subject.enProto-Oncogene Proteins
dc.subject.enStage-Specific Embryonic Antigens
dc.subject.enStem Cell Niche
dc.subject.enStem Cells
dc.title.enIsolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties.
dc.title.alternativeCellsen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cells8121485en_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
dc.identifier.pubmed31766521 - 33922877en_US
bordeaux.journalCellsen_US
bordeaux.volume8en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.issue12en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.institutionCNRS
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.description.error<?xml version="1.0" encoding="utf-8"?> <sword:error xmlns:sword="http://purl.org/net/sword/error/" xmlns="http://www.w3.org/2005/Atom" href="http://purl.org/net/sword/error/ErrorBadRequest"> <title>ERROR</title> <updated>2021-06-14T17:00:00+02:00</updated> <author> <name>HAL SWORD API Server</name> </author> <source> <generator uri="https://api.archives-ouvertes.fr/sword" version="1.0">hal@ccsd.cnrs.fr</generator> </source> <summary>Some parameters sent with the request were not understood</summary> <sword:treatment>processing failed</sword:treatment> <sword:verboseDescription>{"duplicate-entry":{"inserm-02870962":{"doi":"1.0"}}}</sword:verboseDescription> <link rel="alternate" href="https://api.archives-ouvertes.fr" type="text/html"/> </sword:error>
hal.exporttrue
workflow.import.sourcepubmed
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Cells&amp;rft.date=2019-11-21&amp;rft.volume=8&amp;rft.issue=12&amp;rft.eissn=2073-4409&amp;rft.issn=2073-4409&amp;rft.au=R%C3%89MY,%20Murielle&amp;FERRARO,%20Francesca&amp;LE%20SALVER,%20Pierre&amp;REY,%20Sylvie&amp;GENOT,%20Elisabeth&amp;rft.genre=article


Fichier(s) constituant ce document

Thumbnail
Nom:
biotis_cells-2021_boeuf.pdf
Taille:
4.435Mo
Format:
PDF
Voir/Ouvrir
Thumbnail
Nom:
license_rdf
Taille:
914octets
Format:
application/rdf+xml
Voir/Ouvrir

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée

Historique des versions

VersionItemDateEn bref
220.500.12278/79129.22021-06-14T15:04:22ZErratum of Cells 2019, 8(12), 1485
120.500.12278/79129*2021-06-14T14:59:56Z

*Version sélectionnée