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Qualification of labeled Endothelial Progenitor cells for tracking in the context of tissue engineering

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHÉBAUD, Noelie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAUSSEL, Audrey
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorTOUTAIN, Jérôme
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorMONTEMBAULT, Alexandra
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorDAVID, Laurent
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierCIC-IT Bordeaux
dc.contributor.authorBORDENAVE, Laurence
dc.date.accessioned2021-06-10T07:05:55Z
dc.date.available2021-06-10T07:05:55Z
dc.date.issued2015-03-27
dc.date.conference2015-03-25
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79052
dc.description.abstractEnIn order to track location and distribution of endothelial cells (ECs) within scaffolds, we chose lentiPGK-TdTomato transduction of human Endothelial Progenitor Cells (EPCs). Because transduction could have a functional impact on cell behaviour, we checked different parameters for qualification of labeled-EPCs. After isolation and expansion, EPCs were transduced with the lentiviral vector containing the TdTomato protein gene. Conventional karyotyping, differentiation capacity, viability, proliferation assays and functional assays were performed with labeled and unlabeled EPCs. Results show that cell labeling did not affect cell adhesion nor induce cell death. Cell labeling did not induce more chromosomal aberrations. Phenotypical characterization was not affected. In the context of tissue engineering applications, labeled EPCs maintained their ability to line scaffolds, withstand physiological arterial shear stress and form tubular networks in co-cultures with human osteoblast progenitor cells. So it is possible to label human EPCs with TdTomato without affecting their behaviour by the transduction procedure. This creates an important tool for vascular and bone tissue engineering.
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.source.titleActes des Journées RITS 2015
dc.subject.enBiomaterials
dc.subject.enMolecular Imaging
dc.subject.enOptical Imaging
dc.title.enQualification of labeled Endothelial Progenitor cells for tracking in the context of tissue engineering
dc.typeCommunication dans un congrès avec actes
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale
bordeaux.pagepp 108-109
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.countryFR
bordeaux.title.proceedingJournées RITS 2015
bordeaux.conference.cityDourdan
bordeaux.peerReviewedoui
hal.identifierinserm-01154946
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-01154946v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Actes%20des%20Journ%C3%A9es%20RITS%202015&rft.date=2015-03-27&rft.spage=pp%20108-109&rft.epage=pp%20108-109&rft.au=TH%C3%89BAUD,%20Noelie&AUSSEL,%20Audrey&SIADOUS,%20Robin&TOUTAIN,%20J%C3%A9r%C3%B4me&BAREILLE,%20Reine&rft.genre=proceeding


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