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Labeling and qualification of endothelial progenitor cells for tracking in tissue engineering: An in vitro study

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHEBAUD, Noelie B.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAUSSEL, Audrey
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorTOUTAIN, Jérôme
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorMONTEMBAULT, Alexandra
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorDAVID, Laurent
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBORDENAVE, Laurence
dc.date.accessioned2021-06-10T07:05:52Z
dc.date.available2021-06-10T07:05:52Z
dc.date.issued2015-04
dc.identifier.issn0391-3988
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79047
dc.description.abstractEnPurpose: In order to track location and distribution of endothelial cells (ECs) within scaffolds in vitro, we chose lentiPGK-TdTomato transduction of human endothelial progenitor cells (EPCs) isolated and differentiated from cord blood. Because transduction could have a functional impact on cell behavior, we checked different parameters for qualification of labeled-EPCs as well as their use for potential applications in the context of vascular and bone tissue engineering.Methods: After isolation and expansion, EPCs were classically characterized then transduced with the lentiviral vector containing the TdTomato protein gene under the control of the phosphoglycerate kinase (PGK) promoter. Conventional karyotyping, differentiation capacity, viability, proliferation assays were performed with labeled and unlabeled EPCs. Scaffolds and co-cultures were explored with labeled EPCs, in static or shear stress conditions.Results: Our results show that cell labeling did not affect cell adhesion nor induce cell death. Cell labeling did not induce more chromosomal aberrations. Phenotypical characterization was not affected. In the context of tissue engineering applications, labeled EPCs maintained their ability to line 2D or 3D scaffolds, withstand physiological arterial shear stress, and form tubular networks in co-cultures with human osteoblast progenitor cells.Conclusions: It is possible to label human EPCs with TdTomato without affecting their behavior by the transduction procedure. This creates an important tool for numerous applications. Our results provide a qualification of labeled EPCs in comparison with unlabeled ones for vascular and bone tissue engineering.
dc.language.isoen
dc.publisherWichtig Editore
dc.subject.enEndothelial progenitor cells
dc.subject.enLabeled cells
dc.subject.enLentiviral transduction
dc.subject.enCell tracking
dc.subject.enTissue engineering
dc.title.enLabeling and qualification of endothelial progenitor cells for tracking in tissue engineering: An in vitro study
dc.typeArticle de revue
dc.identifier.doi10.5301/ijao.5000405
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
bordeaux.journalInternational Journal of Artificial Organs
bordeaux.page224-232
bordeaux.volume38
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-01221627
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01221627v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International%20Journal%20of%20Artificial%20Organs&rft.date=2015-04&rft.volume=38&rft.spage=224-232&rft.epage=224-232&rft.eissn=0391-3988&rft.issn=0391-3988&rft.au=THEBAUD,%20Noelie%20B.&AUSSEL,%20Audrey&SIADOUS,%20Robin&TOUTAIN,%20J%C3%A9r%C3%B4me&BAREILLE,%20Reine&rft.genre=article


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