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Cell and tissue responses at the interface with a chitosan hydrogel intended for vascular applications: in vitro and in vivo exploration

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale
dc.contributor.authorAUSSEL, Audrey
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOIZIAU, Claudine
ORCID: 0000-0002-0475-2571
IDREF: 85228370
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL AZOU, Beatrice
ORCID: 0000-0001-6725-7108
IDREF: 60910860
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierCIC Bordeaux
dc.contributor.authorDELMOND, Samantha
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorMONTEMBAULT, Alexandra
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorDAVID, Laurent
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCIC Bordeaux
dc.contributor.authorBORDENAVE, Laurence
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale
dc.contributor.authorTHÉBAUD, Noëlie-Brunehilde
dc.date.accessioned2021-06-10T07:05:07Z
dc.date.available2021-06-10T07:05:07Z
dc.date.issued2019-01
dc.identifier.issn1748-6041
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79006
dc.description.abstractEnAims: The need for small caliber vessels to treat cardiovascular diseases has grown. However, synthetic polymers perform poorly in small-diameter applications. Chitosan hydrogels can provide a novel biological scaffold for vascular engineering. The goal of this study was to explore host cell and tissue behavior at the interface with chitosan-based scaffolds in vitro and in vivo.Methods and results: in vitro, we assessed the ability of endothelial cells lining chitosan hydrogels to produce tissue factor (TF), thrombomodulin (TM) and nitric oxide. We showed that endothelial cells behave as a native endothelium since under stimulation, TF and TM expression increased and decreased, respectively. Endothelial cells seeded on chitosan produced nitric oxide, but no change was observed under stimulation. After in vivo subcutaneous implantation of chitosan hydrogels in rats, macrophage activation phenotypes, playing a crucial role in biomaterial/tissue, were explored by immunohistochemistry. Our results suggested a balance between pro- and anti-inflammatory signals since we observed an inflammatory response in favor of macrophage M2 phenotype.Conclusion: in vitro exploration of endothelial cell response at the interface with chitosan hydrogel showed a functional endothelium and in vivo exploration of tissue response revealed a biointegration of chitosan hydrogels.
dc.language.isoen
dc.publisherIOP Publishing
dc.title.enCell and tissue responses at the interface with a chitosan hydrogel intended for vascular applications: in vitro and in vivo exploration
dc.typeArticle de revue
dc.identifier.doi10.1088/1748-605X/aafbf0
dc.subject.halChimie/Matériaux
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Biomatériaux
bordeaux.journalBiomedical Materials
bordeaux.page025009
bordeaux.volume14
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue2
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-02337428
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02337428v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomedical%20Materials&rft.date=2019-01&rft.volume=14&rft.issue=2&rft.spage=025009&rft.epage=025009&rft.eissn=1748-6041&rft.issn=1748-6041&rft.au=AUSSEL,%20Audrey&BOIZIAU,%20Claudine&L%20AZOU,%20Beatrice&SIADOUS,%20Robin&DELMOND,%20Samantha&rft.genre=article


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