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Comparison of the impact of preservation methods on amniotic membrane properties for tissue engineering applications

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFENELON, Mathilde
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorB MAUREL, Delphine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGREMARE, Agathe
hal.structure.identifierPlateforme Technologique d'Innovation Biomédicale [PTIB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDELMOND, Samantha
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDURAND, Marlène
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBRUN, Stéphanie
hal.structure.identifierEcole Dentaire
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
hal.structure.identifierNanomédecine, imagerie, thérapeutique - UFC (UR 4662) [NIT / NANOMEDECINE]
dc.contributor.authorGINDRAUX, Florelle
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL'HEUREUX, Nicolas
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
dc.date.accessioned2021-06-10T07:04:13Z
dc.date.available2021-06-10T07:04:13Z
dc.date.issued2019-11
dc.identifier.issn0928-4931
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78959
dc.description.abstractEnHuman amniotic membrane (hAM) is considered as an attractive biological scaffold for tissue engineering. For this application, hAM has been mainly processed using cryopreservation, lyophilization and/or decellularization. However, no study has formally compared the influence of these treatments on hAM properties. The aim of this study was to develop a new decellularization-preservation process of hAM, and to compare it with other conventional treatments (fresh, cryopreserved and lyophilized). The hAM was decellularized (D-hAM) using an enzymatic method followed by a detergent decellularization method, and was then lyophilized and gamma-sterilized. Decellularization was assessed using DNA staining and quantification. D-hAM was compared to fresh (F-hAM), cryopreserved (C-hAM) and lyophilized/gamma-sterilized (L-hAM) hAM. Their cytotoxicity on human bone marrow mesenchymal stem cells (hBMSCs) and their biocompatibility in a rat subcutaneous model were also evaluated. The protocol was effective as judged by the absence of nuclei staining and the residual DNA lower than 50 ng/mg. Histological staining showed a disruption of the D-hAM architecture, and its thickness was 84% lower than fresh hAM (p < 0.001). Despite this, the labeling of type IV and type V collagen, elastin and laminin were preserved on D-hAM. Maximal force before rupture of D-hAM was 92% higher than C-hAM and L-hAM (p < 0.01), and D-hAM was 37% more stretchable than F-hAM (p < 0.05). None of the four hAM were cytotoxic, and D-hAM was the most suitable scaffold for hBMSCs proliferation. Finally, D-hAM was well integrated in vivo. In conclusion, this new hAM decellularization process appears promising for tissue engineering applications.
dc.language.isoen
dc.publisherElsevier
dc.subject.enAcellular scaffold
dc.subject.enAmniotic membrane
dc.subject.enCryopreservation
dc.subject.enFreeze-drying
dc.subject.enProcessed amnion
dc.subject.enRat
dc.subject.enin vivo biocompatibility
dc.title.enComparison of the impact of preservation methods on amniotic membrane properties for tissue engineering applications
dc.typeArticle de revue
dc.identifier.doi10.1016/j.msec.2019.109903
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalMaterials Science and Engineering: C
bordeaux.page109903
bordeaux.volume104
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870477
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870477v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Materials%20Science%20and%20Engineering:%20C&amp;rft.date=2019-11&amp;rft.volume=104&amp;rft.spage=109903&amp;rft.epage=109903&amp;rft.eissn=0928-4931&amp;rft.issn=0928-4931&amp;rft.au=FENELON,%20Mathilde&amp;B%20MAUREL,%20Delphine&amp;SIADOUS,%20Robin&amp;GREMARE,%20Agathe&amp;DELMOND,%20Samantha&amp;rft.genre=article


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