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Layer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierUniversity of Novi Sad
dc.contributor.authorGUDURIC, Vera
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMETZ, Carole
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierInstitute for Bioengineering of Catalonia [Barcelona] [IBEC]
hal.structure.identifierUniversity Medical Center [Utrecht]
dc.contributor.authorLEVATO, Riccardo
hal.structure.identifierInstitute for Bioengineering of Catalonia [Barcelona] [IBEC]
dc.contributor.authorENGEL, Elisabeth
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDEVILLARD, Raphael
hal.structure.identifierUniversity of Novi Sad
dc.contributor.authorLUZANIN, Ognjan
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
dc.date.accessioned2021-06-10T07:04:00Z
dc.date.available2021-06-10T07:04:00Z
dc.date.issued2017-05
dc.identifier.issn0957-4530
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78947
dc.description.abstractEnThe conventional tissue engineering is based on seeding of macroporous scaffold on its surface ("top-down" approach). The main limitation is poor cell viability in the middle of the scaffold due to poor diffusion of oxygen and nutrients and insufficient vascularization. Layer-by-Layer (LBL) bioassembly is based on "bottom-up" approach, which considers assembly of small cellularized blocks. The aim of this work was to evaluate proliferation and differentiation of human bone marrow stromal cells (HBMSCs) and endothelial progenitor cells (EPCs) in two and three dimensions (2D, 3D) using a LBL assembly of polylactic acid (PLA) scaffolds fabricated by 3D printing. 2D experiments have shown maintain of cell viability on PLA, especially when a co-cuture system was used, as well as adequate morphology of seeded cells. Early osteoblastic and endothelial differentiations were observed and cell proliferation was increased after 7 days of culture. In 3D, cell migration was observed between layers of LBL constructs, as well as an osteoblastic differentiation. These results indicate that LBL assembly of PLA layers could be suitable for BTE, in order to promote homogenous cell distribution inside the scaffold and gene expression specific to the cells implanted in the case of co-culture system.
dc.language.isoen
dc.publisherSpringer Verlag
dc.title.enLayer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering
dc.typeArticle de revue
dc.identifier.doi10.1007/s10856-017-5887-6
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Materials Science: Materials in Medicine
bordeaux.page78
bordeaux.volume28
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue5
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870626
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870626v1
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