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In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDEVILLARD, Raphael
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRÉMY, M.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKALISKY, J.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOURGET, J.-M.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKÉROURÉDAN, O.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, R.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, R.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAMÉDÉE-VILAMITJANA, J.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCHASSANDE, O.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, J.-C.
dc.date.accessioned2021-06-10T07:03:58Z
dc.date.available2021-06-10T07:03:58Z
dc.date.issued2017-01
dc.identifier.issn0143-2885
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78945
dc.description.abstractEnAim: To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.Methodology: A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t-tests and the Mann-Whitney U test were performed.Results: The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).Conclusions: The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
dc.language.isoen
dc.publisherWiley
dc.subject.enadult stem cells
dc.subject.encell differentiation
dc.subject.enendodontics
dc.subject.enhydrogel
dc.subject.enregenerative medicine
dc.subject.entissue engineering.
dc.title.enIn vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics
dc.typeArticle de revue
dc.identifier.doi10.1111/iej.12591
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalInternational Endodontic Journal
bordeaux.page48-57
bordeaux.volume50
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue1
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870711
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870711v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=International%20Endodontic%20Journal&amp;rft.date=2017-01&amp;rft.volume=50&amp;rft.issue=1&amp;rft.spage=48-57&amp;rft.epage=48-57&amp;rft.eissn=0143-2885&amp;rft.issn=0143-2885&amp;rft.au=DEVILLARD,%20Raphael&amp;R%C3%89MY,%20M.&amp;KALISKY,%20J.&amp;BOURGET,%20J.-M.&amp;K%C3%89ROUR%C3%89DAN,%20O.&amp;rft.genre=article


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