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hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSILVA, Diana Isabel
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSANTOS, Bruno Paiva Dos
hal.structure.identifierLaboratoire du Futur [LOF]
dc.contributor.authorLENG, Jacques
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorOLIVEIRA, Hugo
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAMÉDÉE, Joëlle
dc.date.accessioned2021-06-10T07:03:57Z
dc.date.available2021-06-10T07:03:57Z
dc.date.issued2017-12
dc.identifier.issn2041-4889
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78944
dc.description.abstractEnInnervation by the sensory nervous system plays a key role in skeletal development and in orchestration of bone remodeling and regeneration. However, it is unclear how and in which bone cells can sensory nerves act to control these processes. Here, we show a microfluidic coculture system comprising dorsal root ganglion (DRG) neurons and mesenchymal stem cells (MSCs) that more faithfully represents the in vivo scenario of bone sensory innervation. We report that DRG neurons promote the osteogenic differentiation capacity of MSCs, by mediating the increase of alkaline phosphatase activity and the upregulation of osteoblast-specific genes. Furthermore, we show that DRG neurons have a positive impact on Cx43 levels in MSCs during osteoblastogenesis, especially at an early stage of this process. Conversely, we described a negative impact of DRG neurons on MSCs N-cadherin expression at a later stage. Finally, we demonstrate a cytoplasmic accumulation of β-catenin translocation into the nucleus, and subsequently Lymphoid Enhancer Binding Factor 1-responsive transcriptional activation of downstream genes in cocultured MSCs. Together, our study provides a robust body of evidence that the direct interaction of DRG neurons with MSCs in a bone-like microenvironment leads to an enhancement of osteoblast differentiation potential of MSCs. The osteogenic effect of DRG neurons on MSCs is mediated through the regulation of Cx43 and N-cadherin expression and activation of the canonical/β-catenin Wnt signaling pathway.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enDorsal root ganglion neurons regulate the transcriptional and translational programs of osteoblast differentiation in a microfluidic platform
dc.typeArticle de revue
dc.identifier.doi10.1038/s41419-017-0034-3
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalCell Death and Disease
bordeaux.page3209
bordeaux.volume8
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue12
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870932
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870932v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cell%20Death%20and%20Disease&rft.date=2017-12&rft.volume=8&rft.issue=12&rft.spage=3209&rft.epage=3209&rft.eissn=2041-4889&rft.issn=2041-4889&rft.au=SILVA,%20Diana%20Isabel&SANTOS,%20Bruno%20Paiva%20Dos&LENG,%20Jacques&OLIVEIRA,%20Hugo&AM%C3%89D%C3%89E,%20Jo%C3%ABlle&rft.genre=article


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