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CD154 Induces Matrix Metalloproteinase-9 Secretion in Human Podocytes

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierService de Néphrologie-transplantation-dialyse [Bordeaux]
dc.contributor.authorRIGOTHIER, Claire
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDACULSI, Richard
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLEPREUX, Sébastien
hal.structure.identifierLaboratoire Angiogenèse et Micro-environnement des Cancers [LAMC]
dc.contributor.authorAUGUSTE, Patrick
hal.structure.identifierHoward Hughes Medical Institute [HHMI]
hal.structure.identifierDepartment of Molecular & Cell Biology [Berkeley]
hal.structure.identifierCentre for Genomic Regulation - Centre de Regulació Genòmica [Barcelona] [CRG]
dc.contributor.authorVILLENEUVE, Julien
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorDEWITTE, Antoine
hal.structure.identifierInstitut des Maladies Neurodégénératives [Bordeaux] [IMN]
dc.contributor.authorDOUDNIKOFF, Evelyne
hal.structure.identifierUniversity of Bristol [Bristol]
dc.contributor.authorSALEEM, Moin
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOURGET, Chantal
hal.structure.identifierService de Néphrologie-Transplantation-Dialyse
dc.contributor.authorCOMBE, Christian
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRIPOCHE, Jean
dc.date.accessioned2021-06-10T07:03:44Z
dc.date.available2021-06-10T07:03:44Z
dc.date.issued2016-12
dc.identifier.issn0730-2312
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78932
dc.description.abstractEnMatrix remodeling is a key feature of glomerulosclerosis secondary to diabetes or hypertension. Podocytes contribute to glomerular basement membrane (GBM) turnover by producing matrix components and matrix remodelling enzymes, including matrix metalloproteinases (MMPs). The CD40/CD154 signaling pathway modulates matrix remodeling through the synthesis of MMPs and tissue inhibitors of MMPs. Platelets are a primary blood reservoir of CD154. Here we studied, the impact of the CD154/CD40 pathway on MMP-9 expression by cultured human podocytes. The role of CD40/CD154 was evaluated upon exposure of podocytes to recombinant human CD154 (rhCD154) or activated platelet supernatants from healthy human subjects. We first showed by protein and mRNA expression that CD40 was synthesized by podocytes and detectable on kidney tissue sections. CD40 expression was acquired during podocyte differentiation and enhanced upon exposure to rhCD154. In podocytes, rhCD154 induced an increase of MMP-9 production as shown by RT-PCR, Western blot and and gelatin zymography. Activated platelet supernatants induced MMP-9 mRNA synthesis in podocytes, an effect reduced by anti-CD40 antibody. Our results underscore a potential role for platelets through the CD40/CD154 signaling pathway in the control of GBM synthesis and degradation, via its regulatory role on MMP-9 production. CD154 secretion by activated platelets may contribute to GBM alterations in proteinuric nephropathies.
dc.language.isoen
dc.publisherWiley
dc.title.enCD154 Induces Matrix Metalloproteinase-9 Secretion in Human Podocytes
dc.typeArticle de revue
dc.identifier.doi10.1002/jcb.25571
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Cellular Biochemistry
bordeaux.page2737-2747
bordeaux.volume117
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue12
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870968
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870968v1
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