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Parabolic, Flight-Induced, Acute Hypergravity and Microgravity Effects on the Beating Rate of Human Cardiomyocytes

hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorACHARYA, Aviseka
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorBRUNGS, Sonja
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorLICHTERFELD, Yannick
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorHESCHELER, Jürgen
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorHEMMERSBACH, Ruth
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOEUF, Hélène
ORCID: 0000-0002-3006-8773
IDREF: 03205453X
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorSACHINIDIS, Agapios
dc.date.accessioned2021-06-10T07:03:44Z
dc.date.available2021-06-10T07:03:44Z
dc.date.issued2019-04
dc.identifier.issn2073-4409
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78931
dc.description.abstractEnFunctional studies of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hCMs) under different gravity conditions contribute to aerospace medical research. To study the effects of altered gravity on hCMs, we exposed them to acute hypergravity and microgravity phases in the presence and absence of the β-adrenoceptor isoprenalin (ISO), L-type Ca2+ channel (LTCC) agonist Bay-K8644, or LTCC blocker nifedipine, and monitored their beating rate (BR). These logistically demanding experiments were executed during the 66th Parabolic Flight Campaign of the European Space Agency. The hCM cultures were exposed to 31 alternating hypergravity, microgravity, and hypergravity phases, each lasting 20-22 s. During the parabolic flight experiment, BR and cell viability were monitored using the xCELLigence real-time cell analyzer Cardio Instrument®. Corresponding experiments were performed on the ground (1 g), using an identical set-up. Our results showed that BR continuously increased during the parabolic flight, reaching a 40% maximal increase after 15 parabolas, compared with the pre-parabolic (1 g) phase. However, in the presence of the LTCC blocker nifedipine, no change in BR was observed, even after 31 parabolas. We surmise that the parabola-mediated increase in BR was induced by the LTCC blocker. Moreover, the increase in BR induced by ISO and Bay-K8644 during the pre-parabola phase was further elevated by 20% after 25 parabolas. This additional effect reflects the positive impact of the parabolas in the absence of both agonists. Our study suggests that acute alterations of gravity significantly increase the BR of hCMs via the LTCC.
dc.language.isoen
dc.publisherMDPI
dc.subject.encardiomyocytes
dc.subject.enadrenoceptor agonist
dc.subject.enL-type Ca2+ channels
dc.subject.enIsoprenaline
dc.subject.enBay-K8644
dc.subject.enhuman induced pluripotent stem cells
dc.subject.enhypergravity
dc.subject.enmicrogravity.
dc.title.enParabolic, Flight-Induced, Acute Hypergravity and Microgravity Effects on the Beating Rate of Human Cardiomyocytes
dc.typeArticle de revue
dc.identifier.doi10.3390/cells8040352
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalCells
bordeaux.page352
bordeaux.volume8
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue4
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870973
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870973v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.date=2019-04&rft.volume=8&rft.issue=4&rft.spage=352&rft.epage=352&rft.eissn=2073-4409&rft.issn=2073-4409&rft.au=ACHARYA,%20Aviseka&BRUNGS,%20Sonja&LICHTERFELD,%20Yannick&HESCHELER,%20J%C3%BCrgen&HEMMERSBACH,%20Ruth&rft.genre=article


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