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Bioactive micropatterning of biomaterials for induction of endothelial progenitor cell differentiation: Acceleration of in situ endothelialization

hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierDépartement de Génie des Mines, de la Métallurgie et des Matériaux [Quebec, Canada]
dc.contributor.authorROYER, Caroline
hal.structure.identifierCHU de Québec–Université Laval
dc.contributor.authorGUAY‐BÉGIN, Andrée‐Anne
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHANSEAU, Christel
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierCentre de Recherche sur les Matériaux Avancés [Quebec, Canada] [CERMA]
dc.contributor.authorCHEVALLIER, Pascale
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBORDENAVE, Laurence
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierCentre de Recherche sur les Matériaux Avancés [Quebec, Canada] [CERMA]
dc.contributor.authorLAROCHE, Gaetan
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie‐Christine
dc.date.accessioned2021-06-10T07:03:41Z
dc.date.available2021-06-10T07:03:41Z
dc.date.issued2020-07
dc.identifier.issn1549-3296
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78928
dc.description.abstractEnSynthetic grafts do not provide an appealing surface for endothelial cells to adhere and colonize the inner surface. To promote in situ endothelialization the following aspect has to be taken into account, endothelial progenitor cells (EPCs) needs to be mobilized on the surface of the graft. The surface of the graft has to be sufficiently biocompatible to create a prone environment for the EPCs to adhere, proliferate and, differentiate to form a layer and subsequently improve graft patency. In this work, two active molecules GRGDS and sitagliptin, were chosen for their abilities to recruit, enhance adhesion and induce differentiation of endothelial progenitor cells. They were grafted on PET surfaces in order to provide restrained cues triggering cell alignment and evaluate the influence of such structuration on EPCs fate. We then analyze cell behavior onto functionalized biomaterials. Their abilities to control EPCs fate were demonstrated via RT-qPCR, immunofluorescence, and enzymatic tests. The GRGDS/sitagliptin 100 × 10 surface enables to reduce the stemness phenotype on EPCs and induce the expression of endothelial lineage markers. These results highlight the importance of spatial patterning cues in guiding EPCs organization and function, which may have clinical relevance in the development of vascular grafts that promote patency.
dc.language.isoen
dc.publisherWiley
dc.subject.ensurface functionalization
dc.subject.enendothelial progenitor cells
dc.subject.enendothelialization
dc.subject.enin situ differentiation
dc.subject.enpeptides grafting
dc.title.enBioactive micropatterning of biomaterials for induction of endothelial progenitor cell differentiation: Acceleration of in situ endothelialization
dc.typeArticle de revue
dc.identifier.doi10.1002/jbm.a.36918
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Biomedical Materials Research Part A
bordeaux.page1479-1492
bordeaux.volume108
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue7
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870992
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870992v1
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