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Remote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics

dc.contributor.authorLIU, Liwang
dc.contributor.authorVIEL, Alexis
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSAUX, Guillaume
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPLAWINSKI, Laurent
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorMUGGIOLU, Giovanna
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorBARBERET, Philippe
IDREF: 078137748
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPEREIRA, Marco
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorAYELA, Cedric
IDREF: 125917287
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSEZNEC, Herve
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie-Christine
dc.contributor.authorOLIVE, Jean-Marc
dc.contributor.authorAUDOIN, Bertrand
dc.date.accessioned2021-05-14T09:43:05Z
dc.date.available2021-05-14T09:43:05Z
dc.date.issued2019
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/76772
dc.description.abstractEnCell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited by optics and an out-of-plane accuracy down to a tenth of the optical wavelength. For this, GHz coherent acoustic phonons and their resonance harmonics were tracked by means of an ultrafast opto-acoustic technique. After illustrating the measurement accuracy with cell-mimetic polymer films we map the 3D morphology of an entire osteosarcoma cell. The resulting image complies with the image obtained by standard atomic force microscopy, and both reveal very close roughness mean values. In addition, while scanning macrophages and monocytes, we demonstrate an enhanced contrast of thickness mapping by taking advantage of the detection of high-frequency resonance harmonics. Illustrations are given with the remote quantitative imaging of the nucleus thickness gradient of migrating monocyte cells.
dc.language.isoen
dc.title.enRemote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-019-42718-5
dc.subject.halPhysique [physics]
bordeaux.journalSci.Rep
bordeaux.page6409
bordeaux.volume9
bordeaux.hal.laboratoriesInstitut de Mécanique et d’Ingénierie de Bordeaux (I2M) - UMR 5295*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.institutionINRAE
bordeaux.institutionArts et Métiers
bordeaux.peerReviewedoui
hal.identifierhal-02123347
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02123347v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Sci.Rep&rft.date=2019&rft.volume=9&rft.issue=1&rft.spage=6409&rft.epage=6409&rft.au=LIU,%20Liwang&VIEL,%20Alexis&SAUX,%20Guillaume&PLAWINSKI,%20Laurent&MUGGIOLU,%20Giovanna&rft.genre=article


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