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Study of single or mixture 1 of tethered peptide on surfaces to promote hMSCs differentiation toward osteoblastic lineage

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPADIOLLEAU, L.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHANSEAU, Christel
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorDURRIEU, S.
dc.contributor.authorCHEVALLIER, P.
dc.contributor.authorLAROCHE, G.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie‐Christine
dc.date.accessioned2020-04-10T08:41:18Z
dc.date.available2020-04-10T08:41:18Z
dc.date.issued2018
dc.identifier.issn2576-6422en_US
dc.identifier.other10.1021/acsabm.8b00236en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4207
dc.description.abstractEnThe commitment and differentiation of human mesenchymal stem cells (hMSCs) are guided by bioactive molecules within the extracellular matrix. Among the various approaches to design biomaterials, the functionalization of biomaterial surfaces with peptides from the sequence of proteins from the extracellular matrix is quite common. The purpose of this functionalization is to recruit hMSCs and promote their differentiation into the appropriate lineage. The aim of this work was to investigate the influence of RGD and FHRRIKA peptides and peptide sequences taken from bone morphogenic protein (BMP-2) and histone H4 (osteogenic growth peptide; OGP) either tethered alone or as a mixture on the surface of a model material and to also examine the level of hMSC osteogenic commitment without using a differentiation medium. Grafting of the different peptides was assessed by X-ray photoelectron spectroscopy (XPS), while their surface density was quantified by fluorescence microscopy, and their surface properties were assessed by atomic force microscopy (AFM) and contact angle (CA). The osteogenic commitment of hMSCs cultured on the different surfaces was characterized by immunohistochemistry using Runx-2 as an earlier osteogenic marker and OPN, a late osteogenic marker, and by RT-qPCR through the expression of ColI-a1, Runx-2, and ALP. Biological results show that the osteogenic commitment of the hMSCs was increased on surfaces tethered with a mixture of peptides. Results indicate that tethered peptides in the range of pmol mm–2 were indeed effective in inducing a cellular response after 2 weeks of cell culture without using an osteogenic media. These findings contribute to the research efforts to design biomimetic materials able to induce a response in human stem cells through tethered bioactive molecules for bone tissue engineering.
dc.language.isoENen_US
dc.subject.enstem cells
dc.subject.enbiomimetic materials
dc.subject.enbone tissue engineering
dc.subject.enmimetic peptides
dc.subject.ensurface modification
dc.title.enStudy of single or mixture 1 of tethered peptide on surfaces to promote hMSCs differentiation toward osteoblastic lineage
dc.title.alternativeACS Appl. Bio Mater.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acsabm.8b00236en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalACS Applied Bio Materialsen_US
bordeaux.page1800-1809en_US
bordeaux.volume1en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248
bordeaux.issue6en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02539516
hal.version1
hal.date.transferred2020-04-10T08:41:22Z
hal.exporttrue
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