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Antidiarrheal Action of Bacillus subtilis CU1 CNCM I-2745 and Lactobacillus plantarum CNCM I-4547 in Mice

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorURDACI, Maria C.
ORCID: 0000-0002-0868-0050
IDREF: 033307679
dc.contributor.authorLEFEVRE, M.
dc.contributor.authorLAFFORGUE, G.
dc.contributor.authorCARTIER, C.
dc.contributor.authorRODRIGUEZ, B.
dc.contributor.authorFIORAMONTI, J.
dc.date.accessioned2020-04-08T10:18:44Z
dc.date.available2020-04-08T10:18:44Z
dc.date.issued2018
dc.identifier.issn1664-302Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4171
dc.description.abstractEnPreventive actions of probiotics as antidiarrheal agents are well documented, but their mechanisms are poorly understood. Two selected probiotics, Bacillus subtilis CU1 and Lactobacillus plantarum CNCM I-4547, were tested in mouse experimental models of diarrhea and the possible mechanisms of action were investigated. Diarrhea was induced in mice by oral castor oil administration or by i.v. injection of lipopolysaccharide (LPS) of Salmonella enteritis. The antidiarrheal drug loperamide was used as control. Fecal water excretion was quantified for 2 h and paracellular permeability and electrical parameters of the colon were assessed in Ussing chambers. The expression of colonic exchangers or channels and of Toll-like receptor 4 (TLR4) was assessed by immunohistochemistry. Prophylactic treatment with B. subtilis CU1 or with L. plantarum CNCM I-4547 reduced LPS-induced diarrhea. The reduction of water excretion was in the same range as those induced by loperamide. In the castor oil model, this effect was only observed with B. subtilis CU1. The two probiotic treatments abolished the increase in paracellular permeability induced by LPS, but not by castor oil. However, only L. plantarum CNCM I-4547 treatment decreased the colonic expression of TLR-4. After B. subtilis CU1, colonic expression of cystic fibrosis transmembrane conductance regulator (CFTR) was reduced and that of Na+/H+ exchanger 3 (NHE3) increased. B. subtilis CU1 may increase the capacity of the colon to absorb excess of water in diarrheic conditions by acting on CFTR and NHE3 expression. The two probiotics strains showed an impact on diarrhea through limitation of water excretion that may involve paracellular permeability or electrolyte transport for L. plantarum CNCM I-4547 and B. subtilis CU1 respectively.
dc.language.isoENen_US
dc.title.enAntidiarrheal Action of Bacillus subtilis CU1 CNCM I-2745 and Lactobacillus plantarum CNCM I-4547 in Mice
dc.title.alternativeFront. Microbiol.,en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3389/fmicb.2018.01537en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalFrontiers in Microbiologyen_US
bordeaux.page1537en_US
bordeaux.volume9en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02536742
hal.version1
hal.date.transferred2020-04-08T10:18:48Z
hal.exporttrue
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