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Dissecting the biochemical and transcriptomic effects of a locally applied heat treatment on developing Cabernet Sauvignon grape berries

dc.rights.licenseopenen_US
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLECOURIEUX, Fatma
IDREF: 158692306
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorKAPPEL, Christian
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorPIERI, Philippe
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorCHARON, Justine
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorPILLET, Jérémy
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGODINAUD, Ghislaine
IDREF: 225640449
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorRENAUD, Christel
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGOMES, Eric
IDREF: 102208093
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorDELROT, Serge
IDREF: 058711503
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLECOURIEUX, David
IDREF: 061722464
dc.date.accessioned2020-04-02T14:14:36Z
dc.date.available2020-04-02T14:14:36Z
dc.date.issued2017
dc.identifier.issn1664-462Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4083
dc.description.abstractEnReproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development. HT (+ 8°C) was locally applied to clusters from Cabernet Sauvignon fruiting cuttings at three different developmental stages (middle green, veraison and middle ripening). Samples were collected 1, 7, and 14 days after treatment and used for metabolic and transcriptomic analyses. The results showed dramatic and specific biochemical and transcriptomic changes in heat exposed berries, depending on the developmental stage and the stress duration. When applied at the herbaceous stage, HT delayed the onset of veraison. Heating also strongly altered the berry concentration of amino acids and organic acids (e.g., phenylalanine, γ-aminobutyric acid and malate) and decreased the anthocyanin content at maturity. These physiological alterations could be partly explained by the deep remodeling of transcriptome in heated berries. More than 7000 genes were deregulated in at least one of the nine experimental conditions. The most affected processes belong to the categories "stress responses," "protein metabolism" and "secondary metabolism," highlighting the intrinsic capacity of grape berries to perceive HT and to build adaptive responses. Additionally, important changes in processes related to "transport," "hormone" and "cell wall" might contribute to the postponing of veraison. Finally, opposite effects depending on heating duration were observed for genes encoding enzymes of the general phenylpropanoid pathway, suggesting that the HT-induced decrease in anthocyanin content may result from a combination of transcript abundance and product degradation.
dc.language.isoENen_US
dc.subjectMicroclimat
dc.subjectchangement climatique
dc.subjectVigne
dc.subjectVitis Vinifera
dc.subjectBaie de raisin
dc.subjectTraitement thermique
dc.subject.enBerry Development
dc.subject.enClimate Change
dc.subject.enGrapevine
dc.subject.enHigh Temperature
dc.subject.enMetabolomics/Metabolite Profiling
dc.subject.enMicroarrays
dc.subject.enMicroclimate
dc.title.enDissecting the biochemical and transcriptomic effects of a locally applied heat treatment on developing Cabernet Sauvignon grape berries
dc.title.alternativeFront. plant sci.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3389/fpls.2017.00053
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed28197155en_US
bordeaux.journalFrontiers in Plant Scienceen_US
bordeaux.page1-23en_US
bordeaux.volume8en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03170618
hal.version1
hal.date.transferred2021-03-16T11:24:12Z
hal.exporttrue
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