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Roles of dicer-like proteins 2 and 4 in intra- and intercellular antiviral silencing
dc.rights.license | open | en_US |
dc.contributor.author | QIN, Cheng | |
dc.contributor.author | LI, Bin | |
dc.contributor.author | FAN, Yaya | |
dc.contributor.author | ZHANG, Xian | |
dc.contributor.author | YU, Zhiming | |
dc.contributor.author | RYABOV, Eugene | |
dc.contributor.author | ZHAO, Mei | |
dc.contributor.author | WANG, Hui | |
dc.contributor.author | SHI, Nongnong | |
dc.contributor.author | ZHANG, Pengcheng | |
dc.contributor.author | JACKSON, Stephen | |
dc.contributor.author | TÖR, Mahmut | |
dc.contributor.author | CHENG, Qi | |
dc.contributor.author | LIU, Yule | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | GALLUSCI, Philippe | |
dc.contributor.author | HONG, Yiguo | |
dc.date.accessioned | 2020-04-01T12:38:46Z | |
dc.date.available | 2020-04-01T12:38:46Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0032-0889 | en_US |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/4050 | |
dc.description.abstractEn | RNA silencing is an innate antiviral mechanism conserved in organisms across kingdoms. Such a cellular defense involves DICER or DICER-LIKEs (DCLs) that process plant virus RNAs into viral small interfering RNAs (vsiRNAs). Plants encode four DCLs that play diverse roles in cell-autonomous intracellular virus-induced RNA silencing (known as VIGS) against viral invasion. VIGS can spread between cells. However, the genetic basis and involvement of vsiRNAs in non-cell-autonomous intercellular VIGS remains poorly understood. Using GFP as a reporter gene together with a suite of DCL RNAi transgenic lines, here we show that despite the well-established activities of DCLs in intracellular VIGS and vsiRNA biogenesis, DCL4 acts to inhibit intercellular VIGS whereas DCL2 is required (likely along with DCL2-processed/dependent vsiRNAs and their precursor RNAs) for efficient intercellular VIGS trafficking from epidermal to adjacent cells. DCL4 imposed an epistatic effect on DCL2 to impede cell-to-cell spread of VIGS. Our results reveal previously unknown functions for DCL2 and DCL4 that may form a dual defensive frontline for intra- and intercellular silencing to double-protect cells from virus infection in Nicotiana benthamiana. | |
dc.language.iso | EN | en_US |
dc.subject | Biogénèse | |
dc.subject | Infection virale | |
dc.subject | Arn | |
dc.subject | Nicotiana Benthamiana | |
dc.title.en | Roles of dicer-like proteins 2 and 4 in intra- and intercellular antiviral silencing | |
dc.title.alternative | Plant physiol. | en_US |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.1104/pp.17.00475 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | en_US |
dc.identifier.pubmed | 28455401 | en_US |
bordeaux.journal | Plant Physiology | en_US |
bordeaux.page | 1067-1081 | en_US |
bordeaux.volume | 174 | en_US |
bordeaux.hal.laboratories | Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287 | en_US |
bordeaux.issue | 2 | en_US |
bordeaux.institution | Bordeaux Sciences Agro | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
hal.identifier | hal-03170486 | |
hal.version | 1 | |
hal.date.transferred | 2021-03-16T10:17:23Z | |
hal.export | true | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Physiology&rft.date=2017&rft.volume=174&rft.issue=2&rft.spage=1067-1081&rft.epage=1067-1081&rft.eissn=0032-0889&rft.issn=0032-0889&rft.au=QIN,%20Cheng&LI,%20Bin&FAN,%20Yaya&ZHANG,%20Xian&YU,%20Zhiming&rft.genre=article |
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