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The grapevine uncharacterized intrinsic protein 1 (VvXIP1) is regulated by drought stress and transports glycerol, hydrogen peroxide, heavy metals but not water

dc.rights.licenseopenen_US
dc.contributor.authorNORONHA, Henrique
dc.contributor.authorARAÚJO, Diogo
dc.contributor.authorCONDE, Carlos
dc.contributor.authorMARTINS, Ana P.
dc.contributor.authorSOVERAL, Graça
dc.contributor.authorCHAUMONT, François
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorDELROT, Serge
IDREF: 058711503
dc.contributor.authorGERÓS, Hernâni
dc.date.accessioned2020-03-29T14:16:00Z
dc.date.available2020-03-29T14:16:00Z
dc.date.issued2016
dc.identifier.issn1932-6203en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4025
dc.description.abstractEnA MIP (Major Intrinsic Protein) subfamily called Uncharacterized Intrinsic Proteins (XIP) was recently described in several fungi and eudicot plants. In this work, we cloned a XIP from grapevine, VvXIP1, and agrobacterium-mediated transformation studies in Nicotiana benthamiana revealed that the encoded aquaporin shows a preferential localization at the endoplasmic reticulum membrane. Stopped-flow spectrometry in vesicles from the aqy-null yeast strain YSH1172 overexpressing VvXIP1 showed that VvXIP1 is unable to transport water but is permeable to glycerol. Functional studies with the ROS sensitive probe CM-H2DCFDA in intact transformed yeasts showed that VvXIP1 is also able to permeate hydrogen peroxide (H2O2). Drop test growth assays showed that besides glycerol and H2O2, VvXIP1 also transports boric acid, copper, arsenic and nickel. Furthermore, we found that VvXIP1 transcripts were abundant in grapevine leaves from field grown plants and strongly repressed after the imposition of severe water-deficit conditions in potted vines. The observed downregulation of VvXIP1 expression in cultured grape cells in response to ABA and salt, together with the increased sensitivity to osmotic stress displayed by the aqy-null yeast overexpressing VvXIP1, corroborates the role of VvXIP1 in osmotic regulation besides its involvement in H2O2 transport and metal homeostasis.
dc.language.isoENen_US
dc.subjectAgrobacterium
dc.subjectVitis Vinifera
dc.subjectNicotiana Benthamiana
dc.subjectAquaporine
dc.subjectLevure
dc.subjectPeroxyde d'hydrogène
dc.title.enThe grapevine uncharacterized intrinsic protein 1 (VvXIP1) is regulated by drought stress and transports glycerol, hydrogen peroxide, heavy metals but not water
dc.typeArticle de revueen_US
dc.identifier.doi10.1371/journal.pone.0160976en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalPLoS ONEen_US
bordeaux.page18 p.en_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue8en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02523725
hal.version1
hal.date.transferred2020-03-29T14:16:05Z
hal.exporttrue
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