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Remote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics

dc.rights.licenseopenen_US
dc.contributor.authorLIU, Liwang
dc.contributor.authorVIEL, Alexis
IDREF: 223823309
dc.contributor.authorLE SAUX, Guillaume
dc.contributor.authorPLAWINSKI, Laurent
dc.contributor.authorMUGGIOLU, Giovanna
dc.contributor.authorBARBERET, Philippe
IDREF: 078137748
dc.contributor.authorPEREIRA, Marco
dc.contributor.authorAYELA, Cedric
IDREF: 125917287
dc.contributor.authorSEZNEC, Herve
dc.contributor.authorDURRIEU, Marie-Christine
dc.contributor.authorOLIVE, Jean-Marc
IDREF: 06039563X
dc.contributor.authorAUDOIN, Bertrand
dc.date.accessioned2020-02-20T16:42:44Z
dc.date.available2020-02-20T16:42:44Z
dc.date.issued2019-04-23
dc.identifier.issn2045-2322en_US
dc.identifier.otherhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41598-019-42718-5/MediaObjects/41598_2019_42718_MOESM1_ESM.docxen_US
dc.identifier.urioai:crossref.org:10.1038/s41598-019-42718-5
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/3636
dc.description.abstractEnCell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited by optics and an out-of-plane accuracy down to a tenth of the optical wavelength. For this, GHz coherent acoustic phonons and their resonance harmonics were tracked by means of an ultrafast opto-acoustic technique. After illustrating the measurement accuracy with cell-mimetic polymer films we map the 3D morphology of an entire osteosarcoma cell. The resulting image complies with the image obtained by standard atomic force microscopy, and both reveal very close roughness mean values. In addition, while scanning macrophages and monocytes, we demonstrate an enhanced contrast of thickness mapping by taking advantage of the detection of high-frequency resonance harmonics. Illustrations are given with the remote quantitative imaging of the nucleus thickness gradient of migrating monocyte cells.
dc.language.isoENen_US
dc.sourcecrossref
dc.subject.enAcoustics
dc.subject.enImaging
dc.subject.enImaging techniques
dc.subject.enPhotoacoustics
dc.titleRemote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41598-019-42718-5
dc.subject.halSciences de l'ingénieur [physics]/Autre
bordeaux.journalScientific Reportsen_US
bordeaux.page12p.en_US
bordeaux.volume9en_US
bordeaux.hal.laboratoriesLaboratoire d’Intégration du Matériau au Système (IMS) - UMR 5218en_US
bordeaux.issue1en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-03173367
hal.version1
hal.date.transferred2021-03-18T13:13:37Z
hal.exportfalse
workflow.import.sourcedissemin
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