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dc.rights.licenseopenen_US
dc.contributor.authorLOUNNAS, M.
dc.contributor.authorDIACK, A.
dc.contributor.authorNICOL, M. P.
dc.contributor.authorEYANGOH, S.
dc.contributor.authorWOBUDEYA, E.
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorMARCY, Olivier
dc.contributor.authorGODREUIL, S.
dc.contributor.authorBONNET, M.
dc.date.accessioned2021-02-04T13:39:17Z
dc.date.available2021-02-04T13:39:17Z
dc.date.issued2020
dc.identifier.issn1472-9792en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/26130
dc.description.abstractEnStool samples are alternatives to respiratory samples for bacteriological confirmation of childhood tuberculosis but require intensive laboratory processing before molecular testing to remove PCR inhibitors and debris. We aimed to develop a centrifuge-free processing method for use in resource-limited settings based on a sucrose-flotation method that showed good sensitivity for childhood tuberculosis diagnosis. In an in vitro study using Xpert MTB/RIF Ultra on stool samples spiked with defined bacterial concentrations of Mycobacterium tuberculosis (MTB), we compared different simplification parameters to the reference sucrose-flotation method. Best methods were selected based on the rate of invalid/error results and on sensitivity, compared to the reference method on stools spiked at 103 colony forming units (CFU)/g MTB. For final selection, we tested the best parameter combinations at 102 CFU/g. Out of 13 different parameter combinations, three were tested at 102 CFU/g. The best combination used 0.5 g stool, manual shaking, no filtration, 30-min sedimentation, and a 1:3.6 dilution ratio. This method gave 10% invalid/error results and a sensitivity of 70% vs 63% at 103 CFU/g and 53% vs 58% at 102 CFU/g compared to the reference method. This pre-clinical study was able to develop a centrifuge-free processing method to facilitate stool Xpert Ultra testing.
dc.language.isoENen_US
dc.subjectIDLIC
dc.title.enLaboratory development of a simple stool sample processing method diagnosis of pediatric tuberculosis using Xpert Ultra
dc.title.alternativeTuberculosis (Edinb)en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.tube.2020.102002en_US
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologieen_US
bordeaux.journalTuberculosisen_US
bordeaux.page102002en_US
bordeaux.volume125en_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - UMR 1219en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.teamIDLICen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03131547
hal.version1
hal.date.transferred2021-02-04T13:39:21Z
hal.exporttrue
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